Sacran Hydrogel Film Containing Keratinocyte Growth Factor Accelerates Wound Healing by Stimulating Fibroblast Migration and Re-epithelization

Chemical & Pharmaceutical Bulletin
Nasrul WathoniHidetoshi Arima

Abstract

Regenerative therapy with keratinocyte growth factor (KGF) is a novel therapeutic approach for treatment of chronic wounds. However, KGF cannot be used directly to the wound site due to its physicochemical instability. In previous study, sacran, a natural megamolecular polysaccharide, showed potential properties as a biomaterial for hydrogel film in wound healing. In this study, we fabricated sacran hydrogel film containing KGF (Sac/KGF-HF) and evaluated the effects of Sac/KGF-HF on fibroblasts migration and re-epithelialization process. We successfully prepared a homogenous and -amorphous Sac/KGF-HF by a casting method. In addition, Sac/KGF-HF had a high swelling ratio and flexibility. Sac/KGF-HF promoted a migration process of NIH3T3 cells and improved wound healing ability in mice with a percentage of wound closure reaching 90.4% at 9 d. Interestingly, the addition of KGF in Sac-HF considerably increased the number of epithelial cells compared to control, which is important in the re-epithelialization process. It could be concluded that KGF in Sac-HF has the potential for promoting Sac-HF abilities in wound healing process.

References

Apr 1, 1992·The Journal of Surgical Research·M H GartnerM D Caldwell
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A MansukhaniC Basilico
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J S RubinS A Aaronson
Sep 1, 1993·The Journal of Experimental Medicine·L Staiano-CoicoM R Madden
Jul 5, 2003·Physiological Reviews·Sabine Werner, Richard Grose
Feb 10, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Robert F Diegelmann, Melissa C Evans
Apr 7, 2006·The Journal of Biological Chemistry·Xiuqin ZhangDavid M Ornitz
Oct 31, 2006·The Journal of Investigative Dermatology. Symposium Proceedings·Xiao-Jing WangAllen Guanqun Li
Apr 17, 2007·The Journal of Investigative Dermatology·Richard A F ClarkMarcia G Tonnesen
May 9, 2007·Frontiers in Bioscience : a Journal and Virtual Library· RajaR Rivkah Isseroff
Nov 3, 2007·Advances in Skin & Wound Care·Cathy BurrowsDavid H Keast
Nov 3, 2007·Biomaterials·Chayanin PratoomsootFelicity Rosamari A J Rose
Dec 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ileana Ruxandra BotusanSergiu-Bogdan Catrina
Jan 9, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Stephan BarrientosMarjana Tomic-Canic
Apr 15, 2009·Drug Development and Industrial Pharmacy·Joshua S BoatengKerr H Matthews
Feb 9, 2010·Journal of Dental Research·S Guo, L A Dipietro
Aug 20, 2010·Journal of the Royal Society, Interface·Kangwon LeeDavid J Mooney
Sep 30, 2010·Lab on a Chip·S T KoevR Ghodssi
Apr 13, 2012·Perspectives in Vascular Surgery and Endovascular Therapy·Elisavet K TiakaGeorge S Georgiadis
Apr 17, 2012·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Piyush Koria
Feb 28, 2013·Nature Reviews. Molecular Cell Biology·João V Cordeiro, António Jacinto
Jul 18, 2014·Advances in Wound Care·Irena PastarMarjana Tomic-Canic
Mar 26, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Garazi GainzaManoli Igartua
May 7, 2015·Advances in Wound Care·Mark A Seeger, Amy S Paller
May 7, 2016·International Journal of Biological Macromolecules·Nasrul WathoniHidetoshi Arima
May 15, 2016·Carbohydrate Polymers·MeeiChyn GohGiyoong Tae
Feb 6, 2017·International Journal of Biological Macromolecules·Nasrul WathoniHidetoshi Arima
Feb 28, 2017·Journal of Advanced Research·Elbadawy A KamounXin Chen
Jul 28, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jin Woo ParkIn-Soo Yoon
Sep 5, 2018·Carbohydrate Polymers·Suhela TyebVivek Verma

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Citations

Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lisa Efriani PuluhulawaNasrul Wathoni

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