SadB is required for the transition from reversible to irreversible attachment during biofilm formation by Pseudomonas aeruginosa PA14

Journal of Bacteriology
Nicky C Caiazza, George A O'Toole

Abstract

Current models of biofilm formation by Pseudomonas aeruginosa propose that (i) planktonic cells become surface associated in a monolayer, (ii) surface-associated cells form microcolonies by clonal growth and/or aggregation, (iii) microcolonies transition to a mature biofilm comprised of exopolysaccharide-encased macrocolonies, and (iv) cells exit the mature biofilm and reenter the planktonic state. Here we report a new class of P. aeruginosa biofilm mutant that defines the transition from reversible to irreversible attachment and is thus required for monolayer formation. The transposon insertion carried by the sadB199 mutant was mapped to open reading frame PA5346 of P. aeruginosa PA14 and encodes a protein of unknown function. Complementation analysis and phage-mediated transduction demonstrated that the transposon insertion in PA5346 was the cause of the biofilm-defective phenotype. Examination of flow cell-grown biofilms showed that the sadB199 mutant could initiate surface attachment but failed to form microcolonies despite being proficient in both twitching and swimming motility. Closer examination of early attachment revealed an increased number of the sadB199 mutant cells arrested at reversible attachment, functionally d...Continue Reading

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Citations

Mar 18, 2009·Annals of Biomedical Engineering·Vijay JanakiramanHarihara Baskaran
Nov 13, 2012·Nucleic Acids Research·Deepak BalasubramanianKalai Mathee
Oct 17, 2012·Antimicrobial Agents and Chemotherapy·John W Lamppa, Karl E Griswold
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Aug 7, 2007·Journal of Bacteriology·Robert M Q ShanksGerard J Nau
Mar 6, 2007·Journal of Bacteriology·Nicky C CaiazzaGeorge A O'Toole
Aug 20, 2005·Journal of Bacteriology·Ernst Weber, Ralf Koebnik
Oct 21, 2005·Journal of Bacteriology·Nicky C CaiazzaG A O'Toole
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·Ece Karatan, Paula Watnick
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