Safety and biological activity evaluation of Uncaria rhynchophylla ethanolic extract.

Drug and Chemical Toxicology
Heung Bin Lim, Hyeong Ryeol Lee

Abstract

Uncaria rhynchophylla (UR) belongs to the Rubiaceae family, and its dried hooks are usually used in traditional medicine. It is effective in treating diseases related to the central nervous system. This study aimed to evaluate the safety of UR extract, investigate its antimutagenic and antioxidative activities, and elucidate its active components. Extraction and fractionation of the UR extract resulted in yields of 6.71%, 0.037%, 0.042%, 0.152%, 0.332%, and 5.132%, for hexane, ether, DCM, EtOAC, and aqueous fractions, respectively. The four indole alkaloids, total phenolic content (TPC), and total flavonoid content (TFC) of UR extract and its subfractions were measured. Alkaloid content was highest in the UR extract. TPC was the highest in the EtOAC fraction (373.7 ± 20.9 mg gallic acid equivalent (GAE)/g), whereas TFC was the highest in the UR extract (33.5 ± 2.4 mg quercetin equivalent (QE)/g). To assess the safety of UR extract mutagenicity, cytotoxicity, and oxidative stress inducibility assays were performed. The UR extract (2000 µg/plate) showed excellent antimutagenic activity (above 90%) against BaP in both TA98 and TA100 strains. The UR extract exhibited efficient DPPH (RC50 239.2 ± 16.5 µg/mL) and ABTS scavenging acti...Continue Reading

References

May 1, 1985·Toxicology and Applied Pharmacology·G F RushW R Hewitt
May 1, 1983·Mutation Research·D M Maron, B N Ames
Jan 1, 1995·Biochemical Society Symposium·C Rice-Evans
Jun 25, 1999·Free Radical Biology & Medicine·R ReC Rice-Evans
Sep 9, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T C HourJ K Lin
Nov 18, 2000·Mutation Research·J L MarnewickE Joubert
Nov 23, 2000·Nature·T Finkel, N J Holbrook
Aug 9, 2002·Journal of Agricultural and Food Chemistry·Veronica DewantoRui Hai Liu
Mar 20, 2004·Nature Reviews. Drug Discovery·Kevin J BarnhamAshley I Bush
May 14, 2005·Journal of Ethnopharmacology·Wen-Chi HouMei-Hsien Lee
Jul 11, 2006·Journal of Ethnopharmacology·Ji Wook JungJong Hoon Ryu
Jul 22, 2006·Life Sciences·Cristovao F LimaCristina Pereira-Wilson
Oct 13, 2006·Biochimica Et Biophysica Acta·Michael Schrader, H Dariush Fahimi
Feb 21, 2007·Journal of Natural Products·David J Newman, Gordon M Cragg
Mar 14, 2007·Chemico-biological Interactions·Cristovao F LimaCristina Pereira-Wilson
Jun 20, 2007·Plant Foods for Human Nutrition·María Elena Santos-CervantesFrancisco Delgado-Vargas
May 23, 2008·The Journal of Nutritional Biochemistry·Ana Belén Granado-SerranoSonia Ramos
Jul 5, 2008·Nature Protocols·Guillermo RepettoJorge L Zurita
Nov 11, 2008·Current Drug Metabolism·L L Custer, K S Sweder
Dec 31, 2009·Phytotherapy Research : PTR·María Angeles MartínLuis Goya
Feb 13, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Maryam ZahinFarrukh Aqil
Apr 27, 2010·Best Practice & Research. Clinical Obstetrics & Gynaecology·Haitham Hamoda, Allan Templeton
Jul 13, 2011·The Journal of Cell Biology·Toren Finkel
Jul 31, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Beatriz SarriáLuis Goya
Mar 13, 2014·Journal of Applied Genetics·Karolina SłoczyńskaAnna M Waszkielewicz
Mar 19, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Tin-Yun HoChing-Liang Hsieh
Nov 5, 2014·Chemical & Pharmaceutical Bulletin·Hai-Bo WangDan Yuan
Jun 21, 2015·Journal of Ethnopharmacology·Qian ZhangWei Qu
Sep 26, 2015·Science·Iñigo Martincorena, Peter J Campbell
Feb 1, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yongliang ZhuangLiping Sun

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