Safety and feasibility of exhaled breath condensate collection in ventilated infants and children

The European Respiratory Journal
W G MullerA Jaffe

Abstract

The aim of this study was to develop a technique for the collection of exhaled breath condensate (EBC) from ventilated children and assess its safety and feasibility. Collection of EBC is used to investigate markers of oxidative stress in the lower airway. No studies have assessed its safety in ventilated children. An in vitro model was developed by connecting a ventilator to an artificial lung; 14 clinical and ventilatory parameters were measured during EBC collection from ventilated children. Levels of 8-isoprostane were measured following collection with and without humidification of the inhaled gas. Amount of water vapour collected was linearly related to time and to minute ventilation in the in vitro model. EBC collections (n = 68) were made from ventilated children. In the nonhumidified group, the mean (range) positive end-expiratory pressure increased by 4.1% (2.8-5.5%) and the peak inspiratory flow decreased by 6.1% (11.0-1.3%) during collection. Detectable levels of 8-isoprostane were only found in 10 out of 18 nonhumidified EBC samples (median (range) 4.7 pg x mL(-1) (0-5.8)). Collection of exhaled breath condensate from ventilated infants and children is feasible and safe. Discontinuation of humidification is likely ...Continue Reading

Citations

Jul 3, 2015·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Fiammetta Piersigilli, Vineet Bhandari
Aug 11, 2012·Immunology and Allergy Clinics of North America·Michael D DavisJohn Hunt
May 23, 2012·Pediatric Pulmonology·Kavita PatelCharles R Esther
Nov 15, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E van MastrigtM W Pijnenburg
Mar 9, 2012·Respiratory Medicine·Stewart R CarterElizabeth J Kovacs
Jul 18, 2017·Pediatric Pulmonology·Olivia Giddings, Charles R Esther
Oct 27, 2007·Pediatric Pulmonology·Christian VogelbergWolfgang Leupold
Jan 23, 2020·American Journal of Critical Care : an Official Publication, American Association of Critical-Care Nurses·Mairi ZiakaEpaminondas Zakynthinos
Jun 6, 2013·Expert Review of Respiratory Medicine·Shannon J SimpsonGraham L Hall
Jul 25, 2018·Journal of Neonatal-perinatal Medicine·E YenM Ramagopal
Oct 31, 2020·ERJ Open Research·Jeppe Hjembaek-BrandtMorten Heiberg Bestle

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