Safety Assessment of Bacteroides uniformis CECT 7771 Isolated from Stools of Healthy Breast-Fed Infants

PloS One
M Leonor Fernández-Murga, Yolanda Sanz

Abstract

Bacteroides uniformis CECT 7771 is a potential probiotic strain, originally isolated from the stools of healthy breast-feed infants. The strain showed pre-clinical efficacy in a mouse obesity model. The objective of this study was to evaluate its potential toxicity and translocation ability after acute oral administration to mice. A safety study was conducted in immunocompetent and immunosuppressed C57BL-6 mice. Both mouse groups (n = 10 per group) were fed orally 2 x 10(9) colony forming units (cfu)/day of B. uniformis CECT 7771 or placebo by gavage for 6 days. Throughout this time, feed and water intake and body weight were monitored. Afterwards, mice were sacrificed and biological samples were collected to analyze blood and urine biochemistry, inflammatory and immune markers; gut mucosal histology and bacterial translocation to peripheral tissues. The results demonstrated that acute ingestion of this Bacteroides strain had no adverse effects on the animals' general health status or food intake, nor did it affect biochemical indicators of liver, kidney and pancreatic function or gut mucosal histology. Findings also demonstrated that administration did not lead to bacterial translocation to blood, liver or mesenteric lymph nod...Continue Reading

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Citations

Sep 2, 2017·Current Opinion in Clinical Nutrition and Metabolic Care·Marina Romaní-PérezYolanda Sanz
Jun 2, 2020·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Sonia FacchinEdoardo Vincenzo Savarino
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Feb 6, 2019·Food Research International·Huizi TanWei Chen
Feb 26, 2021·Molecular Nutrition & Food Research·Alfonso Benítez-PáezUNKNOWN MyNewGut consortium
May 1, 2021·Microorganisms·Xuemei LiuWei Chen
Jun 29, 2021·Current Pharmaceutical Design·Tanya RalliKanchan Kohli

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Methods Mentioned

BETA
enzymatic assay
electrophoresis
PCR
protein
dissection

Software Mentioned

Graph Pad
GraphPad Prism
NIS Elements BR

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