Safety evaluation of an oat grain alkaloid gramine by genotoxicity assays

Drug and Chemical Toxicology
Manash Pratim PathakPronobesh Chattopadhyay

Abstract

Gramine is a natural indole alkaloid that has been isolated from different raw plants occurring mainly in Avena sativa, etc. The study was aimed to investigate the possible in vitro antioxidant, in vitro mutagenic, in vitro antimutagenic, and in vivo genotoxic activity of gramine using ferric reducing ability of plasma (FRAP) assay, Metal chelating, Ames bacterial reverse mutation test, and the mouse bone marrow micronucleus assay as well as chromosomal aberration. Four concentrations of gramine viz. 250, 500, 1000, and 2000 μg/mL were evaluated for its antioxidant activity in FRAP Assay and Metal Chelating Test. Four concentrations of gramine (1250 μg/plate, 2500 μg/plate, 5000 μg/plate, and 10 000 μg/plate) were employed in Salmonella typhimurium strains to study the mutagenicity in the presence and absence of standard mutagens, 2-aminofluorene (2-AF), sodium azide (SA), and 2-nitrofluorene (2-NF). Three doses, i.e. 0.1, 0.2, and 0.3 × the LD50 of gramine (i.e. 50 mg/kg, 100 mg/kg, and 150 mg/kg) were administered orally to either sex of Swiss albino mice for 48 h to study the genotoxic activity in micronucleus assay as well as chromosomal aberration. Gramine showed potent antioxidant activity in both the assay. Gramine at th...Continue Reading

References

Feb 1, 1975·Mutation Research·W Schmid
Feb 1, 1979·Mutation Research·B Gollapudi, O P Kamra
Dec 1, 1990·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·R L Noble
May 1, 1983·Mutation Research·D M Maron, B N Ames
Oct 1, 1984·Biochemical Pharmacology·H M Niemeyer, O A Roveri
Dec 20, 1999·Journal of Agricultural and Food Chemistry·Y IkkenM I Cambero
Dec 13, 2000·Mutation Research·K Mortelmans, E Zeiger
Dec 13, 2000·Mutation Research·G Krishna, M Hayashi
May 30, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F V SantosE A Varanda
Jan 16, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·R S PolicegoudraS M Aradhya
Feb 28, 2008·European Journal of Nutrition·Masood Sadiq ButtMehmood S Butt
Nov 11, 2008·Mutation Research·Seung-Shi HamMyeon Choe
Feb 12, 2009·Cardiovascular Therapeutics·Yiyi SunYitao Wang
Jun 13, 2009·Mini Reviews in Medicinal Chemistry·Fernando Rodrigues de Sá AlvesCarlos Alberto Manssour Fraga
Jun 3, 2011·Nutrition Reviews·Rgia A OthmanPeter Jh Jones
Dec 8, 2011·Methods in Molecular Biology·Ian de G Mitchell, David O F Skibinski
Oct 18, 2012·Critical Reviews in Food Science and Nutrition·Rajinder SinghAsma Belkheir
Jul 31, 2013·Methods in Molecular Biology·Sawako KasamotoMakoto Hayashi
Jul 31, 2013·Methods in Molecular Biology·Gireesh H Kamath, K S Rao
Aug 20, 2015·Marine Drugs·Natalie Netz, Till Opatz
Jun 1, 2011·Journal of Adolescent and Young Adult Oncology·Leonard S Sender
Jun 29, 2016·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Kei-Ichi SugiyamaAtsushi Hakura

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Citations

Oct 31, 2019·Toxicology and Industrial Health·Srijita ChakrabartiPronobesh Chattopadhyay
Mar 20, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Naima ChamlalFabrice Nesslany

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