Safety evaluation of chinese medicine injections with a cell imaging-based multiparametric assay revealed a critical involvement of mitochondrial function in hepatotoxicity

Evidence-based Complementary and Alternative Medicine : ECAM
Meng WangYan Zhu

Abstract

The safety of herbal medicine products has been a widespread concern due to their complex chemical nature and lack of proper evaluation methods. We have adapted a sensitive and reproducible multiparametric cell-based high-content analysis assay to evaluate the hepatic-safety of four Chinese medicine injections and validated it with classical animal-based toxicity assays. Our results suggested that the reported hepatotoxicity by one of the drugs, Fufangkushen injection, could be attributed at least in part to the interference of mitochondrial function in human HepG2 cells by some of its constituents. This method should be useful for both preclinical screen in a drug discovery program and postclinical evaluation of herbal medicine preparations.

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Citations

Nov 15, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yiming XuLisheng Wang

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Methods Mentioned

BETA
MDA

Software Mentioned

SPSS
Graph Pad
Graph Pad Grism
SFDA

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