Safety of poly (ethylene glycol)-coated perfluorodecalin-filled poly (lactide-co-glycolide) microcapsules following intravenous administration of high amounts in rats

Results in Pharma Sciences
Katja B FerenzMichael Kirsch

Abstract

The host response against foreign materials designates the biocompatibility of intravenously administered microcapsules and thus, widely affects their potential for subsequent clinical use as artificial oxygen/drug carriers. Therefore, body distribution and systemic parameters, as well as markers of inflammation and indicators of organ damage were carefully evaluated after administration of short-chained poly (vinyl alcohol, (PVA)) solution or poly (ethylene glycol (PEG))-shielded perfluorodecalin-filled poly (d,l-lactide-co-glycolide, PFD-filled PLGA) microcapsules into Wistar rats. Whereas PVA infusion was well tolerated, all animals survived the selected dose of 1247 mg microcapsules/kg body weight but showed marked toxicity (increased enzyme activities, rising pro-inflammatory cytokines and complement factors) and developed a mild metabolic acidosis. The observed hypotension emerging immediately after start of capsule infusion was transient and mean arterial blood pressure restored to baseline within 70 min. Microcapsules accumulated in spleen and liver (but not in other organs) and partly occluded hepatic microcirculation reducing sinusoidal perfusion rate by about 20%. Intravenous infusion of high amounts of PFD-filled PL...Continue Reading

Citations

Mar 20, 2018·Biomedical Optics Express·Jannis KöhlerAndreas Ostendorf
Nov 7, 2019·European Journal of Applied Physiology·Dirk Mayer, Katja Bettina Ferenz
Nov 4, 2020·Pflügers Archiv : European journal of physiology·Johannes JägersKatja B Ferenz

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BETA
Assay
ELISA
fluorescence microscopy

Software Mentioned

AxioVision
GraphPad
graph pad prism

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