PMID: 7082452Jan 1, 1982Paper

Sagamicin and the related aminoglycosides: fermentation and biosynthesis. I. Biosynthetic studies with the blocked mutants of Micromonospora sagamiensis

The Journal of Antibiotics
H KaseK Nakayama

Abstract

The mutants blocked in the gentamicin C1 production were derived from a sagamicin producing strain of Micromonospora sagamiensis. The intermediates produced by these mutants were isolated and properly identified. Comparing the biotransformation activities in the resting cells of the mutants with those of a DOS idiotroph, KY 11525, the blocked steps in sagamicin and gentamicin biosynthesis were proposed in each mutant. Mutant KY 11564 was found to produce gentamicin C2a (C-6'-epimer of gentamicin C2) together with gentamicin C1a and sagamicin. KY 11525 transformed gentamicin C2a into C2 and C1, whereas KY 11564 lacked the activity. KY 11565 produced gentamicin X2 and antibiotic G-418, and lacked 6'-amino substitution activities. KY 11566 appeared to be partially blocked in 6'-N-methylation activities, and the major products were gentamicin C2a and C1a. From these results, sagamicin biosynthesis in M. sagamiensis is discussed.

Citations

Nov 23, 2006·Natural Product Reports·Nicholas M Llewellyn, Jonathan B Spencer
Oct 11, 2008·Natural Product Research·Chun-Mei XueZhi-Wei Deng
Jun 18, 2015·Chembiochem : a European Journal of Chemical Biology·Yawen GuHuanzhang Xia
Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Je Won ParkYeo Joon Yoon
Oct 22, 2008·Critical Reviews in Biotechnology·C Ganesh KumarAnnapurna Jetty

Related Concepts

XK-62-2
Aminoglycosides
Antibiotics
Metabolic Biotransformation
Fermentation
Gentacycol
Micromonospora
Gumbaral

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Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.