Sal k 4, a new allergen of Salsola kali, is profilin: a predictive value of conserved conformational regions in cross-reactivity with other plant-derived profilins

Bioscience, Biotechnology, and Biochemistry
Mohammad Ali AssarehzadeganAbdolreza Varasteh

Abstract

The aim of this study was to investigate a new allergen of Salsola kali, Sal k 4, and to investigate the predictive value of the conserved conformational regions in cross-reactivity with other plant-derived profilins. The Sal k 4-coding sequence was cloned, expressed, and purified by one-step Ni2+ affinity chromatography to recover high-purity target protein. We assessed cross-reactivity and predicted conserved conformational regions among rSal k 4 and other plant-derived profilins. Immunodetection and inhibition assays using 30 individual sera from S. kali allergic patients indicated that purified rSal k 4 might be the same as that in the crude extract. The results of inhibition assays among rSal k 4 and other plant-derived profilins were in accordance with the homology of the predicted conserved conformational regions. Amino acid sequence homology analysis showed that a high degree of IgE cross-reactivity among plant-derived profilins might depend on the predicted conserved conformational regions.

References

Feb 1, 1992·The Journal of Experimental Medicine·R ValentaD Kraft
Jan 15, 1997·Structure·K S ThornC E Schutt
Apr 1, 1997·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·F A SuliamanR Ratard
Mar 22, 2000·Allergy·C I EzeamuzieZ Hijazi
Apr 18, 2000·Gene·D E Wilkes, J J Otto
Aug 5, 2003·The Journal of Allergy and Clinical Immunology·Riccardo AseroGianni Caldironi
Mar 3, 2004·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Abdulrahman Al-DowaisanIrfan Khan
Sep 13, 2005·Clinical and Molecular Allergy : CMA·Mojtaba SankianMahmoud Mahmoudi
Jul 15, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C RadauerH Breiteneder
Jun 22, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R BarderasM Villalba
Mar 25, 2009·Allergology International : Official Journal of the Japanese Society of Allergology·Mohammad Ali AssarehzadeganAbdolReza Varasteh
May 19, 2009·Allergologia et immunopathologia·Mohammad FereidouniAbdolreza Varasteh
Jul 25, 2009·Allergology International : Official Journal of the Japanese Society of Allergology·Abdol-Reza VarastehMojtaba Sankian

❮ Previous
Next ❯

Citations

Mar 25, 2011·Allergology International : Official Journal of the Japanese Society of Allergology·Mohsen TehraniAbdolreza Varasteh
Jan 4, 2012·International Archives of Allergy and Immunology·Lucía FerrerCarlos Colas
Nov 20, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Mohammad-Hossein ShamsHaleh Abdoli Sereshki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Allergology International : Official Journal of the Japanese Society of Allergology
Mohammad Ali AssarehzadeganAbdolreza Varasteh
Allergology International : Official Journal of the Japanese Society of Allergology
Mohammad Ali AssarehzadeganAbdolreza Varasteh
Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
Rodrigo BarderasM Villalba
© 2021 Meta ULC. All rights reserved