PMID: 7542698May 1, 1995Paper

Salamander olfactory bulb neuronal activity observed by video rate, voltage-sensitive dye imaging. I. Characterization of the recording system

Journal of Neurophysiology
A R CinelliJ S Kauer

Abstract

1. In this paper we describe properties of a video imaging system used to acquire voltage-sensitive dye fluorescence signals from the salamander olfactory bulb. Sources of noise in these signals were evaluated in preparations stained with the potentiometric probe RH-414. These were compared with noise levels in signals obtained from a light-emitting diode array designed to stimulate the experimental conditions with light levels similar to those seen in the salamander bulb recordings. These experiments define a number of determinants of video image quality to standardize optical voltage measurements in the salamander olfactory bulb. 2. Images were acquired at video rates using a Newvicon camera in a standard upright microscope and digitized with an eight-bit video frame grabber. 3. Sources of noise related to camera sensitivity, stability of illumination, and mechanical vibration were characterized. Camera dark noise was less than the pixel variability due to photon noise at the camera faceplate. This pixel noise was the limiting factor for discriminating the spatial and temporal properties of the optical responses. 4. No significant noise was found to be related to image digitization, transmission, or readout by the eight-bit f...Continue Reading

Citations

Apr 30, 1998·Physiological Reviews·D Schild, D Restrepo
Aug 25, 2001·Annual Review of Neuroscience·J S Kauer, J White
Jan 18, 2002·The Journal of Biological Chemistry·Riad EfendievCarlos H Pedemonte
Oct 22, 2004·Nature Reviews. Neuroscience·Amiram Grinvald, Rina Hildesheim
May 7, 2002·Journal of Neuroscience Research·J DuK A Hamilton
May 12, 2007·Journal of Neurophysiology·Michael T LippertJian-Young Wu
Jul 30, 2005·Cellular and Molecular Neurobiology·Bradley J BakerDejan Zecevic
Feb 13, 2004·Journal of Neurophysiology·Rainer W Friedrich, Gilles Laurent
May 1, 1996·Journal of Neurobiology·A GelperinI R Cooke
Mar 6, 2002·Journal of Neurophysiology·Angel R CinelliMimi Halpern
Aug 31, 2000·Methods : a Companion to Methods in Enzymology·A R Cinelli
Jan 6, 2016·Cold Spring Harbor Protocols·Amiram GrinvaldRina Hildesheim

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