Salicylic acid activates poplar defense against the biotrophic rust fungus Melampsora larici-populina via increased biosynthesis of catechin and proanthocyanidins

The New Phytologist
Chhana UllahAlmuth Hammerbacher

Abstract

Poplar trees synthesize flavan-3-ols (catechin and proanthocyanidins) as a defense against foliar rust fungi, but the regulation of this defense response is poorly understood. Here, we investigated the role of hormones in regulating flavan-3-ol accumulation in poplar during rust infection. We profiled levels of defense hormones, signaling genes, and flavan-3-ol metabolites in black poplar leaves at different stages of rust infection. Hormone levels were manipulated by external sprays, genetic engineering, and drought to reveal their role in rust fungal defenses. Levels of salicylic acid (SA), jasmonic acid, and abscisic acid increased in rust-infected leaves and activated downstream signaling, with SA levels correlating closely with those of flavan-3-ols. Pretreatment with the SA analog benzothiadiazole increased flavan-3-ol accumulation by activating the MYB-bHLH-WD40 complex and reduced rust proliferation. Furthermore, transgenic poplar lines overproducing SA exhibited higher amounts of flavan-3-ols constitutively via the same transcriptional activation mechanism. These findings suggest a strong association among SA, flavan-3-ol biosynthesis, and rust resistance in poplars. Abscisic acid also promoted poplar defense against r...Continue Reading

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Citations

Feb 27, 2019·Tree Physiology·Scott A Harding
Nov 26, 2019·Molecular Plant-microbe Interactions : MPMI·Si-Qi TaoSébastien Duplessis
Feb 3, 2021·Journal of Agricultural and Food Chemistry·Aleksandra BurdziejMarie-France Corio-Costet
Nov 25, 2020·Phytochemistry·Jin Zhang, Xiaoling Sun
Jan 1, 2021·Frontiers in Plant Science·Steven DreischhoffAndrea Polle
Mar 23, 2021·Frontiers in Plant Science·Pulimamidi BharathAgepati S Raghavendra
Apr 30, 2021·Evolution; International Journal of Organic Evolution·Karl C FetterStephen R Keller
Dec 15, 2020·Journal of Agricultural and Food Chemistry·Peiqiang WangTao Xia
Sep 15, 2020·Microbiological Research·Manisha PhourBernard R Glick

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Datasets Mentioned

BETA
SRP146333
SRP146326
SRP146339
SRP146322

Methods Mentioned

BETA
transgenic
spore
PCR
reverse transcription PCR

Software Mentioned

mage J
DESeq2
Tophat2
HTSeq

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