Salidroside inhibits the proliferation and migration of gastric cancer cells via suppression of Src‑associated signaling pathway activation and heat shock protein 70 expression

Molecular Medicine Reports
Zhilin QiYao Zhang

Abstract

Salidroside, an active ingredient extracted from the Rhodiola rosea plant, has potential anti‑tumor effects. However, the effects of salidroside on gastric cancer cell proliferation and migration remain unclear. In the present study, the inhibitory effects of salidroside on gastric cancer cell proliferation, migration and invasion and the molecular mechanisms underlying these effects were investigated. The human gastric cancer cell line, BGC‑823, was treated with different concentrations of salidroside (200, 400 and 600 µg/ml). Cell proliferation was determined with Cell Counting Kit‑8 and colony formation assays, and the migration and invasion of cells was detected by a wound healing and Transwell assay, respectively. Western blotting was performed to detect the levels of N‑cadherin, E‑cadherin and heat shock protein (HSP)70. In addition, the phosphorylation of proto‑oncogene tyrosine‑protein kinase Src (Src), protein kinase B (Akt), mitogen activated protein kinase 1 (ERK), signal transducer and activator of transcription (STAT)3 and focal adhesion kinase 1 (FAK) was examined by western blotting. The levels of matrix metalloproteinase (MMP)‑2 and MMP‑9 were determined by enzyme‑linked immunosorbent assay kits. Levels of react...Continue Reading

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Citations

Sep 24, 2020·Drug Development and Industrial Pharmacy·Dan Hong, Hong-Mei Zhao
Jan 2, 2021·International Journal of Molecular Sciences·Joanna KozakJacek Baj
Jul 3, 2021·International Journal of Molecular Sciences·Alina D NikotinaIrina V Guzhova

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
fluorescence microscopy
ELISA

Software Mentioned

SPSS

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