Salidroside-Mediated Autophagic Targeting of Active Src and Caveolin-1 Suppresses Low-Density Lipoprotein Transcytosis across Endothelial Cells

Oxidative Medicine and Cellular Longevity
Xiangli BaiSi Jin

Abstract

Subendothelial retention of apolipoprotein B100-containing lipoprotein, such as low-density lipoprotein (LDL), is the initial step of atherogenesis. Activation of autophagy exhibits beneficial effects for the treatment of atherosclerosis. In our previous study, we demonstrated that hyperglycemia suppressed autophagic degradation of caveolin-1, which in turn resulted in acceleration of caveolae-mediated LDL transcytosis across endothelial cells and lipid retention. Therefore, targeting the crossed pathway in autophagy activation and LDL transcytosis interruption may be a promising antiatherosclerotic strategy. In metabolic diseases, including atherosclerosis, salidroside, a phenylpropanoid glycoside compound (3,5-dimethoxyphenyl) methyl-β-glucopyranoside), is the most important compound responsible for the therapeutic activities of Rhodiola. However, whether salidroside suppresses LDL transcytosis to alleviate atherosclerosis has not yet been elucidated. In the present study, we demonstrated that salidroside significantly decreased LDL transcytosis across endothelial cells. Salidroside-induced effects were dramatically blocked by AMPK (adenosine monophosphate-activated protein kinase) inhibitor (compound c, AMPKα siRNA) and by o...Continue Reading

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Citations

Dec 7, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kai HouXuping Qin
Oct 23, 2020·The Journal of Membrane Biology·Natasha BuwaNagaraj Balasubramanian
Jul 8, 2021·Journal of Cardiovascular Pharmacology·Yibo LiGuoan Zhao

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Methods Mentioned

BETA
flow cytometry
immunoprecipitation
transfection
light microscopy
transmission electron microscopy
fluorescence microscopy
coimmunoprecipitation

Software Mentioned

ImageJ pro plus
ARRIVE
ImageJ

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