Salinity-Aided Selection of Progressive Onset Denitrifiers as a Means of Providing Nitrite for Anammox

Environmental Science & Technology
Wei LiJames P Shapleigh

Abstract

Anaerobic ammonium oxidation (anammox) combined with partial-denitrification (NO3- → NO2-) is an innovative process for the simultaneous removal of ammonia and nitrate from wastewaters. An efficient method for the selection of partial denitrifying community, which relies on increasing influent salinity, is described. Using this method, a denitratating community was enriched, which showed a nitrite accumulation efficiency higher than 75% as well as a high nitrate conversion efficiency. Community analysis using 16S rDNA indicated that Halomonas became the dominant genus as salinity increased. Metagenomic analysis revealed that there was not a significant difference in reads mapping to downstream denitrification genes in a comparison of samples from cultures with 5% salinity to those without salinity. The majority of the reads mapping to the genes encoding dissimilatory nitrate and nitrite reductases nar and nirS came from Halomonas under high salinity conditions. Two metagenome-assembled genomes taxonomically assigned to Halomonas were obtained, one of which accounted for ∼35% of the reads under high salinity conditions. Both genomes harbored the genes for the complete denitrification pathway. These results indicate progressive o...Continue Reading

References

Mar 5, 2004·Environmental Science & Technology·Kris PynaertWilly Verstraete
Jun 6, 2006·Water Science and Technology : a Journal of the International Association on Water Pollution Research·K FurukawaT Fujii
Oct 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael Richter, Ramon Rosselló-Móra
Mar 10, 2010·BMC Bioinformatics·Doug HyattLoren J Hauser
Mar 28, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Lars R BakkenAsa Frostegård
May 15, 2013·Environmental Microbiology·Binbin LiuAsa Frostegård
Nov 18, 2014·Nature Methods·Benjamin BuchfinkDaniel H Huson
Jul 8, 2015·Nucleic Acids Research·Neha J VargheseAmrita Pati
Nov 4, 2015·Applied Microbiology and Biotechnology·Rui DuMeng Niu
Feb 26, 2016·Environmental Microbiology·Constance A RocoJames P Shapleigh
Apr 1, 2016·Nature Communications·Daan R SpethMike S M Jetten
Oct 7, 2016·Applied Microbiology and Biotechnology·Wei LiPing Zheng
Mar 18, 2017·Environmental Science & Technology·Ananda S BhattacharjeeRamesh Goel
Jun 1, 2017·Nature Communications·Christopher E LawsonDaniel R Noguera
Jul 12, 2017·The ISME Journal·Pawel LycusÅsa Frostegård
Sep 18, 2017·Metabolic Engineering·Xiao-Ran JiangGuo-Qiang Chen

❮ Previous
Next ❯

Citations

Jul 16, 2020·Environmental Science and Pollution Research International·Yaqi XieNana Lu
Sep 21, 2019·Environmental Science & Technology·Shenbin CaoYan Zhou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.