Abstract
Salinomycin (SAL), a monocarboxylic polyether antibiotic isolated from Streptomyces albus, modulates various cellular responses, including proliferation, apoptosis, and inflammation. However, the effect of SAL on the dedifferentiation of chondrocytes remains unclear. Thus, we investigated the effects and regulatory mechanisms of SAL on the dedifferentiation of rabbit articular chondrocytes. Our results indicate that SAL-induced a loss of type II collagen and decreased sulfated proteoglycan levels in a dose- and time-dependent manner, as assessed by western blot analysis and alcian blue staining. Consistent with dedifferentiation, we found that type II collagen expression was decreased and type I collagen and SOX-9 expression was increased using RT-PCR. Immunohistochemical and immunofluorescence staining also indicated dedifferentiation of chondrocytes. SAL treatment activated the mitogen-activated protein (MAP) kinase signaling pathway. Among the MAP kinases, extracellular signal-regulated kinase (ERK) was phosphorylated and translocated into the nucleus from the cytosol following SAL treatment. Inhibition of ERK with PD98059 (PD) rescued the SAL-induced decrease in type II collagen, increase in type I collagen, and reduction i...Continue Reading
References
Mar 1, 1992·Molecular and Cellular Biology·R H ChenJ Blenis
Sep 1, 1993·The Journal of Cell Biology·P LenormandJ Pouysségur
Sep 1, 2000·Osteoarthritis and Cartilage·A M DeLiseR S Tuan
Nov 2, 2001·The Journal of Biological Chemistry·Song-Ja KimJang-Soo Chun
Dec 18, 2001·The Journal of Biological Chemistry·Young-Mee YoonJang-Soo Chun
Oct 31, 2002·Development·Je-Hwang RyuJang-Soo Chun
Aug 5, 2003·BioFactors·Martine Torres, Henry Jay Forman
Aug 9, 2003·The Journal of Biological Chemistry·Song-Ja KimJang-Soo Chun
Sep 9, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Peter S BurrageConstance E Brinckerhoff
Oct 29, 2005·Cancer Letters·Florence A SchollPaul A Khavari
May 6, 2008·Best Practice & Research. Clinical Rheumatology·Johanne Martel-PelletierPeter J Roughley
Jan 31, 2009·Arthritis and Rheumatism·Arjen B BlomWim B van den Berg
Oct 20, 2009·Biochemical and Biophysical Research Communications·Dominik FuchsCord Naujokat
Nov 3, 2010·Osteoarthritis and Cartilage·A W M ChengV B Kraus
Aug 30, 2011·Biochemical and Biophysical Research Communications·Kwang-Youn KimSoon-Cheol Ahn
Dec 8, 2011·Chemical Biology & Drug Design·Adam Huczynski
Mar 15, 2012·The Veterinary Record
Nov 6, 2012·Biochemical and Biophysical Research Communications·Maria Szkudlarek-MikhoKhew-Voon Chin
Dec 20, 2012·Journal of Biomedicine & Biotechnology·Cord Naujokat, Roman Steinhart
Sep 11, 2013·Experimental Cell Research·Seon-Mi Yu, Song-Ja Kim
Citations
Oct 25, 2016·Experimental Cell Research·Seong-Hui EoSong Ja Kim
May 18, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shuaijun LiLei Cui
Aug 22, 2020·Npj Regenerative Medicine·Yongchang Yao, Chunming Wang
Feb 13, 2021·Npj Regenerative Medicine·Yongchang Yao, Chunming Wang
Jan 2, 2018·International Immunopharmacology·Jian WuXian-Hua Cai
Dec 1, 2021·Immunopharmacology and Immunotoxicology·Xuegang SunShengwei He