Salinomycin induces endoplasmic reticulum stress‑mediated autophagy and apoptosis through generation of reactive oxygen species in human glioma U87MG cells

Oncology Reports
Sun-Nyoung YuSoon-Cheol Ahn

Abstract

Salinomycin is a polyether ionophore antibiotic that has recently been shown to induce cell apoptosis in human cancer cells displaying multiple mechanisms of drug resistance. In the present study, we explored the impact of salinomycin on the apoptosis and autophagy as well as the correlation between those effects and endoplasmic reticulum (ER) stress molecular mechanisms in human glioma U87MG cells. Apoptosis, autophagy and reactive oxygen species (ROS) were analyzed using flow cytometry. In addition, expression levels of apoptosis-, autophagy- and ER stress-related proteins were determined by western blotting. The results showed that salinomycin induced apoptosis, ER stress and autophagy in glioma cancer cell lines. In addition, salinomycin also induced ROS generation, and the ROS scavenger N-acetyl-L-cysteine was found to inhibit the salinomycin-induced apoptosis, ER stress and autophagy. The inhibition of ER stress with 4-phenylbutyric acid depressed salinomycin-induced apoptosis and autophagy. Salinomycin increased the expression of autophagy marker protein, LC3B, and accumulation of acidic vesicular organelles. Furthermore, pre-treatment with the autophagy inhibitor 3-methyladenine showed potential in increasing the apopto...Continue Reading

References

Mar 21, 2001·Apoptosis : an International Journal on Programmed Cell Death·H U SimonF Levi-Schaffer
Aug 25, 2004·The International Journal of Biochemistry & Cell Biology·Richard A Lockshin, Zahra Zakeri
Feb 3, 2005·Acta Neuropathologica·Hiroko Ohgaki, Paul Kleihues
Feb 1, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Harmeet MalhiJohn J Lemasters
Sep 6, 2006·EMBO Reports·Eva SzegezdiAfshin Samali
Jan 20, 2007·The Journal of Clinical Investigation·Ravi K AmaravadiCraig B Thompson
Jan 15, 2008·Cell·David Ron, Stevan R Hubbard
Mar 28, 2009·Cell Death and Differentiation·A Eisenberg-LernerA Kimchi
Aug 27, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Eileen White, Robert S DiPaola
Jul 24, 2010·Antioxidants & Redox Signaling·Michel RigouletAnne Devin
Dec 24, 2010·Cancer Biology & Therapy·Paola Maycotte, Andrew Thorburn
Feb 26, 2013·Antioxidants & Redox Signaling·Zhiyong Cheng, Michael Ristow
Jun 5, 2013·Cell Biochemistry and Biophysics·Chun-ling WangXiao-hong Cao
Mar 7, 2015·Free Radical Biology & Medicine·Agnès Delaunay-Moisan, Christian Appenzeller-Herzog
Apr 2, 2016·Biochemical and Biophysical Research Communications·Sang-Hun KimSoon-Cheol Ahn

❮ Previous
Next ❯

Citations

Feb 13, 2018·Journal of Experimental & Clinical Cancer Research : CR·Jiang JiangZeyao Tang
Feb 15, 2019·PloS One·Johannes KloseMartin Schneider
Jul 25, 2019·Antioxidants·Peter F SuraiMichael T Kidd
Jan 6, 2019·European Journal of Medicinal Chemistry·Michał Antoszczak
May 1, 2021·Frontiers in Molecular Biosciences·Shulong CaoHaiyuan Zhang
Jul 3, 2021·International Journal of Molecular Sciences·Maya A DymovaVladimir A Richter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagy & Metabolism

Autophagy preserves the health of cells and tissues by replacing outdated and damaged cellular components with fresh ones. In starvation, it provides an internal source of nutrients for energy generation and, thus, survival. A powerful promoter of metabolic homeostasis at both the cellular and whole-animal level, autophagy prevents degenerative diseases. It does have a downside, however--cancer cells exploit it to survive in nutrient-poor tumors.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.