Abstract
Salinomycin is a polyether ionophore antibiotic that has recently been shown to induce cell apoptosis in human cancer cells displaying multiple mechanisms of drug resistance. In the present study, we explored the impact of salinomycin on the apoptosis and autophagy as well as the correlation between those effects and endoplasmic reticulum (ER) stress molecular mechanisms in human glioma U87MG cells. Apoptosis, autophagy and reactive oxygen species (ROS) were analyzed using flow cytometry. In addition, expression levels of apoptosis-, autophagy- and ER stress-related proteins were determined by western blotting. The results showed that salinomycin induced apoptosis, ER stress and autophagy in glioma cancer cell lines. In addition, salinomycin also induced ROS generation, and the ROS scavenger N-acetyl-L-cysteine was found to inhibit the salinomycin-induced apoptosis, ER stress and autophagy. The inhibition of ER stress with 4-phenylbutyric acid depressed salinomycin-induced apoptosis and autophagy. Salinomycin increased the expression of autophagy marker protein, LC3B, and accumulation of acidic vesicular organelles. Furthermore, pre-treatment with the autophagy inhibitor 3-methyladenine showed potential in increasing the apopto...Continue Reading
References
Mar 21, 2001·Apoptosis : an International Journal on Programmed Cell Death·H U SimonF Levi-Schaffer
Aug 25, 2004·The International Journal of Biochemistry & Cell Biology·Richard A Lockshin, Zahra Zakeri
Feb 3, 2005·Acta Neuropathologica·Hiroko Ohgaki, Paul Kleihues
Feb 1, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Harmeet MalhiJohn J Lemasters
Jun 24, 2006·Cell Death and Differentiation·Y KourokuT Momoi
Sep 6, 2006·EMBO Reports·Eva SzegezdiAfshin Samali
Jan 20, 2007·The Journal of Clinical Investigation·Ravi K AmaravadiCraig B Thompson
Jan 15, 2008·Cell·David Ron, Stevan R Hubbard
Jul 8, 2008·Brain Research·Arabinda DasSwapan K Ray
Oct 17, 2008·Cancer Research·Yu Sun, Basil Rigas
Mar 28, 2009·Cell Death and Differentiation·A Eisenberg-LernerA Kimchi
Aug 27, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Eileen White, Robert S DiPaola
Jul 24, 2010·Antioxidants & Redox Signaling·Michel RigouletAnne Devin
Dec 24, 2010·Cancer Biology & Therapy·Paola Maycotte, Andrew Thorburn
Jan 27, 2011·Annals of Surgical Oncology·Tao-Tao DongMin-Hua Zheng
Aug 30, 2011·Biochemical and Biophysical Research Communications·Kwang-Youn KimSoon-Cheol Ahn
Sep 23, 2011·Journal of Translational Medicine·Sheng-Hua ChuPu-Cha Jiang
Aug 29, 2012·PLoS Genetics·Robyn D MoirIan M Willis
Jan 4, 2013·PloS One·Fan WangChuan-Yong Guo
Feb 26, 2013·Antioxidants & Redox Signaling·Zhiyong Cheng, Michael Ristow
Mar 19, 2013·Gynecologic Oncology·Soshi KusunokiSatoru Takeda
Apr 26, 2013·Carcinogenesis·Mi Jin YoonKyeong Sook Choi
Jun 5, 2013·Cell Biochemistry and Biophysics·Chun-ling WangXiao-hong Cao
Jun 17, 2014·Oncology Letters·Min LiuXiaodong Liu
Mar 7, 2015·Free Radical Biology & Medicine·Agnès Delaunay-Moisan, Christian Appenzeller-Herzog
May 11, 2015·Peptides·Ju Hye JangJu Hyun Cho
Apr 2, 2016·Biochemical and Biophysical Research Communications·Sang-Hun KimSoon-Cheol Ahn
Apr 29, 2016·Oncotarget·Enric XipellMarta M Alonso
Citations
Dec 15, 2017·Cell & Bioscience·Weiming SunChangqing Xu
Feb 13, 2018·Journal of Experimental & Clinical Cancer Research : CR·Jiang JiangZeyao Tang
Feb 15, 2019·PloS One·Johannes KloseMartin Schneider
Jul 25, 2019·Antioxidants·Peter F SuraiMichael T Kidd
Apr 27, 2019·BMC Cancer·Yunsheng ZhangYi Wang
Oct 16, 2019·Aging·Yaxin HuFeng Han
Nov 6, 2018·JCI Insight·Saurabh AggarwalSadis Matalon
Jul 18, 2020·BMC Cancer·Vanessa BellatBenedict Law
Aug 5, 2017·Journal of Cellular Physiology·Shuling SongQiang Zhang
Jan 6, 2019·European Journal of Medicinal Chemistry·Michał Antoszczak
May 1, 2021·Frontiers in Molecular Biosciences·Shulong CaoHaiyuan Zhang
May 19, 2021·Journal of Dairy Science·Zhen ShiGuowen Liu
May 20, 2019·The Science of the Total Environment·Samwel M LimbuZhen-Yu Du
Jul 3, 2021·International Journal of Molecular Sciences·Maya A DymovaVladimir A Richter