Saliva collection devices affect sex steroid concentrations

Clinica Chimica Acta; International Journal of Clinical Chemistry
Peter Celec, Daniela Ostatníková

Abstract

Saliva collection devices solve some of the technical issues related to sampling and processing of saliva. However, the widely used Salivette cotton or polypropylene rolls interfere with down-stream analysis of some of the salivary compounds. The aim of our study was to prove the effect of sampling using two different Salivette collection devices on salivary testosterone and estradiol concentrations. Saliva samples were collected from young healthy women (n=231) and men (n=69) using Salivette cotton and polypropylene rolls or by passive drooling as whole unstimulated saliva. Testosterone and estradiol concentrations were determined using ELISA. Repeated measures ANOVA and Bland-Altman plots showed that Salivette cotton rolls considerably increase salivary testosterone (by 42% in women and by 28% in men) and estradiol levels (by 9% in women and by 33% in men). Samples collected using the Salivette polypropylene rolls contained significantly lower concentrations of salivary testosterone (by 16%) and estradiol (by 15%) in women, but not in men. Sampling using Salivette collection devices significantly alters salivary concentrations of sex steroids. Although the mechanism of bias introduced by the sampling is not clear, Salivette c...Continue Reading

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Citations

Nov 21, 2013·Progress in Neuro-psychopharmacology & Biological Psychiatry·Omri WeismanRuth Feldman
Feb 7, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Claas GolatowskiUwe Völker
Oct 22, 2013·Domestic Animal Endocrinology·D OyamaG Watanabe
Feb 17, 2015·Hormones and Behavior·Janek S LobmaierMarkus Heinrichs
Nov 8, 2015·Psychoneuroendocrinology·Silja SollbergerUlrike Ehlert
Aug 21, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kuniaki TakagiMasakuni Degawa
Sep 14, 2019·Antioxidants·Jorge M González-HernándezAsta Tvarijonaviciute
Feb 2, 2020·International Journal of Behavioral Medicine·Jenna K RiederMariann R Weierich
Jan 27, 2021·Microbial Biotechnology·Monika JaníkováPeter Celec

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