Salivary bacterial fingerprints of established oral disease revealed by the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) technique

Journal of Oral Microbiology
D BelstrømP Holmstrup

Abstract

The composition of the salivary microbiota, as determined using various molecular methods, has been reported to differentiate oral health from diseases. Thus, the purpose of this study was to utilize the newly developed molecular technique HOMINGS (Human Oral Microbe Identification using Next Generation Sequencing) for comparison of the salivary microbiota in patients with periodontitis, patients with dental caries, and orally healthy individuals. The hypothesis was that this method could add on to the existing knowledge on salivary bacterial profiles in oral health and disease. Stimulated saliva samples (n=30) were collected from 10 patients with untreated periodontitis, 10 patients with untreated dental caries, and 10 orally healthy individuals. Salivary microbiota was analyzed using HOMINGS and statistical analysis was performed using Kruskal-Wallis test with Benjamini-Hochberg's correction. From a total of 30 saliva samples, a mean number of probe targets of 205 (range 120-353) were identified, and a statistically significant higher mean number of targets was registered in samples from patients with periodontitis (mean 220, range 143-306) and dental caries (mean 221, range 165-353) as compared to orally healthy individuals ...Continue Reading

Associated Clinical Trials

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Citations

Nov 20, 2016·American Journal of Infection Control·Livia Barenghi, Alberto Di Blasio
Mar 10, 2018·Journal of Periodontology·Daniel BelstrømPalle Holmstrup
Aug 3, 2017·Journal of Clinical Periodontology·Ignacio Sanz-MartinFlavia R F Teles
Apr 18, 2018·JDR Clinical and Translational Research·D BelstrømP Holmstrup
May 19, 2019·Clinical Oral Implants Research·Xiao-Lin YuRory M Watt
Jan 2, 2019·Journal of Oral Microbiology·Reisha RafeekKaren E Nelson
Apr 15, 2020·Oral Diseases·Sarah F F GuedesLidiany K A Rodrigues
Nov 27, 2019·Journal of the American Heart Association·Charlene E GohRyan T Demmer
Mar 23, 2017·Journal of Oral Microbiology·Daniel BelstrømAllan Bardow
Jul 6, 2019·Journal of Oral Microbiology·Kyrill SchoilewSébastien Boutin
Mar 5, 2020·Journal of Oral Microbiology·Daniel Belstrøm
Jul 28, 2017·Journal of Oral Microbiology·Palle HolmstrupPeter Riis Hansen
Jul 21, 2017·Scientific Reports·Linda ErikssonIngegerd Johansson
Jul 4, 2020·Scientific Reports·Luciana Carla Neves de BritoFlávia Rocha Fonseca Teles
Sep 7, 2019·Journal of Oral Microbiology·Christian DamgaardDaniel Belstrøm
Nov 29, 2020·Journal of Dentistry·Muawia A QudeimatNathanael O Salako
Oct 17, 2020·Journal of Oral Microbiology·Irina M VelskoLuciana Shaddox
Mar 6, 2021·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Xiaoqing LiHongping Zhang
Jul 11, 2021·Archives of Oral Biology·Deesha BhaumikBetsy Foxman
Oct 12, 2021·Frontiers in Cellular and Infection Microbiology·Jing DiaoShuguo Zheng

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Software Mentioned

Graphpad prism
HOMI
QIIME
Probeseq
HOMI NGS
HOMIM
MeV

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