Salivary gel-forming mucin MUC5B--a nutrient for dental plaque bacteria

Oral Microbiology and Immunology
Claes Wickström, Gunnel Svensäter

Abstract

Model systems with oral bacteria from dental plaque have demonstrated that the utilization of complex glycoproteins as a food source cannot be undertaken by single species but requires concerted degradation by a multi-species consortium, with each member contributing one or a few hydrolytic enzymes. Unlike previous studies, the aim of the present investigation was to explore the ability of fresh dental plaque to degrade salivary mucin, MUC5B, isolated by methods designed to retain intact the natural polymeric structure and physiological conformation, in an attempt to mimic the naturally occurring interaction between the oral microbiota and salivary mucins. Human salivary MUC5B was isolated from whole saliva by density-gradient centrifugation and incubated with freshly isolated supragingival dental plaque with samples subjected to fluorescent staining for viability and metabolic activity. In addition, the degradation of MUC5B oligosaccharide side chains was studied using a lectin assay, recognizing three different carbohydrate epitopes commonly found on mucin oligosaccharide side chains. The addition of purified salivary MUC5B elicited a strong metabolic response from the biofilm cells, whereas individual strains of Streptococcu...Continue Reading

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Citations

Jun 2, 2010·Nature Reviews. Microbiology·Paul E KolenbranderNicholas S Jakubovics
Mar 25, 2010·Molecular Oral Microbiology·N S Jakubovics
Sep 10, 2013·BMC Oral Health·Claes WickströmGunnel Svensäter
Nov 4, 2015·Biochimica Et Biophysica Acta·Giovanna BatoniSemih Esin
Dec 22, 2011·Current Opinion in Microbiology·Apollo R StacyMarvin Whiteley
Mar 14, 2012·European Journal of Oral Sciences·Jiang-Wu YaoFeng Lin
Jul 22, 2010·Oral Diseases·N S Jakubovics, P E Kolenbrander
Nov 25, 2011·European Journal of Oral Sciences·Mikael SonessonClaes Wickström
Jul 7, 2011·Molecular Oral Microbiology·V FröjdG Svensäter
Jan 16, 2010·Environmental Microbiology·S R VartoukianW G Wade
Jun 27, 2009·Microbiology·Claes WickströmGunnel Svensäter
Jan 25, 2014·Oral Diseases·B VanhoeckeD Keefe
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Aug 18, 2010·Periodontology 2000·Kazuyuki Ishihara
Feb 7, 2021·Oral Diseases·Massimo FusconiAndrea Gallo
Feb 16, 2021·BMC Microbiology·Gabriella BoisenJessica Neilands
Oct 28, 2020·Journal of Bacteriology·Brittany L NairnMark C Herzberg
Jun 1, 2021·Expert Review of Clinical Immunology·Jin ImaiNobuhiko Kamada
Aug 28, 2021·Journal of Oral Microbiology·Carolina RobertssonClaes Wickström
Nov 18, 2020·Journal of Colloid and Interface Science·Hannah BoydJavier Sotres

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