Salivary Total Protease Activity Based on a Broad-Spectrum Fluorescence Resonance Energy Transfer Approach to Monitor Induction and Resolution of Gingival Inflammation

Molecular Diagnosis & Therapy
Floris J BikkerN Bostanci

Abstract

Salivary total protease and chitinase activities were measured by a broad-spectrum fluorescence resonance energy transfer approach as predictors of induction and resolution of gingival inflammation in healthy individuals by applying an experimental human gingivitis model. Dental biofilm accumulated (21 days, Induction Phase) by omitting oral hygiene practices followed by a 2-week Resolution Phase to restore gingival health in an experimental gingivitis study. Plaque accumulation, as assessed by the Turesky Modification of the Quigley-Hein Plaque Index (TQHPI), and gingival inflammation, assessed using the Modified Gingival Index (MGI), scores were recorded and unstimulated saliva was collected weekly. Saliva was analysed for total protein, albumin, total protease activity and chitinase activity (n = 18). The TQHPI and MGI scores, as well as total protease activity, increased until day 21. After re-establishment of oral hygiene, gingival inflammation levels returned to values similar to baseline (day 0). Levels of protease activity decreased significantly, but not to baseline values. Furthermore, 'fast' responders, who responded immediately to plaque, exhibited significantly higher proteolytic activity throughout the experimenta...Continue Reading

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Citations

Aug 15, 2020·European Journal of Oral Sciences·Dimitri MulkernMelissa M Grant
Aug 11, 2020·Journal of Clinical Periodontology·Nagihan BostanciRodrigo Lopez
Mar 5, 2021·Clinical Oral Investigations·Jeroen VinkePrashant K Sharma
Mar 21, 2021·Scientific Reports·Nagihan BostanciMichal Karpíšek
May 18, 2021·Journal of Clinical Periodontology·Panagiota KatsikiElena A Nicu
Oct 20, 2021·Journal of Clinical Periodontology·Fábio R M LeiteRodrigo López

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Methods Mentioned

BETA
ELISA
Protein Assay
fluorescence
FRET

Software Mentioned

STATA

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