Sall1 transiently marks undifferentiated heart precursors and regulates their fate

Journal of Molecular and Cellular Cardiology
Yuika MoritaJun K Takeuchi

Abstract

Cardiac progenitor cells (CPCs) are a crucial source of cells in cardiac development and regeneration. However, reported CPCs are heterogeneous, and no gene has been identified to transiently mark undifferentiated CPCs throughout heart development. Here we show that Spalt-like gene 1 (Sall1), a zing-finger transcription factor, is expressed in undifferentiated CPCs giving rise to both left and right ventricles. Sall1 was transiently expressed in precardiac mesoderm contributing to the first heart field (left ventricle precursors) but not in the field itself. Similarly, Sall1 expression was maintained in the second heart field (outflow tract/right ventricle precursors) but not in cardiac cells. In vitro, high levels of Sall1 at mesodermal stages enhanced cardiomyogenesis, whereas its continued expression suppressed cardiac differentiation. Our study demonstrates that Sall1 marks CPCs in an undifferentiated state and regulates cardiac differentiation. These findings provide fundamental insights into CPC maintenance, which can be instrumental for CPC-based regenerative medicine.

References

Apr 12, 2001·BMC Developmental Biology·S SrinivasF Costantini
Jun 3, 2004·Mechanisms of Development·Minoru TakasatoRyuichi Nishinakamura
Nov 24, 2005·Nature Reviews. Genetics·Margaret BuckinghamStéphane Zaffran
Dec 19, 2006·Cell·Daniel J Garry, Eric N Olson
Feb 4, 2010·Genesis : the Journal of Genetics and Development·Shuji InoueRyuichi Nishinakamura
Feb 10, 2011·Nature Communications·Jun K TakeuchiBenoit G Bruneau
May 25, 2011·Cardiovascular Research·Jan Hendrick van WeerdJun K Takeuchi
May 23, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Chorom PakShigeki Miyamoto
May 31, 2015·Methods in Molecular Biology·Shin-Il KimKnut Woltjen
Nov 21, 2015·Cell Reports·Hideki UosakiChulan Kwon

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Citations

Jun 13, 2018·The Journal of Biological Chemistry·Eunjin ChoYoungsook Lee
Oct 6, 2018·American Journal of Medical Genetics. Part a·Andreas GiannakouJames L Mills
Aug 9, 2018·Nature Communications·Peter AndersenChulan Kwon
Dec 15, 2018·Development, Growth & Differentiation·Wataru KatanoKazuko Koshiba-Takeuchi
May 31, 2019·Scientific Reports·Masahito WatanabeHiroshi Nagashima
Oct 23, 2020·Circulation Journal : Official Journal of the Japanese Circulation Society·Xiaoxi YangTetsushi Furukawa
May 19, 2021·Progress in Biophysics and Molecular Biology·Abimbola J AminuHalina Dobrzynski
Aug 3, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martha E FloySean P Palecek

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