Salmon calcitonin and amyloid beta: two peptides with amyloidogenic capacity adopt different conformational manifolds in their unfolded states

Biochemistry
Reinhard Schweitzer-StennerKai Griebenow

Abstract

The molecular conformations of salmon calcitonin in aqueous solution have been investigated by exploiting the different influences of excitonic coupling on the amide I band profile in the isotropic and anisotropic Raman, FTIR, and vibrational circular dichroism spectra of a polypeptide. The N-terminal loop, caused by a disulfide bridge between cysteines at positions 1 and 7, was modeled by performing a conformational search by molecular mechanics calculations. The remaining part of the peptide chain was modeled as a mixture of three sequences containing different fractions of residues adopting poly-l-proline II (PPII), extended beta-strand, and alpha-helix-like conformations. This yielded an excellent reproduction of the experimentally observed amide I' band profiles. A comparison with recent data on the beta-amyloid fragment Abeta(1)(-)(28) revealed a lower PPII content and more conformational heterogeneity for calcitonin. Thus, our results underscore the notion that individual structural propensities of amino acid residues give rise to structural differences between the unfolded states of even long peptide chains, at variance with expectations based on a random or statistical coil model.

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Citations

Dec 18, 2009·Journal of the American Chemical Society·Andrew HagarmanReinhard Schweitzer-Stenner
Dec 10, 2009·Journal of the American Chemical Society·Ravi P R NangaAyyalusamy Ramamoorthy
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Wei ZhuangShaul Mukamel
Feb 9, 2007·The Journal of Chemical Physics·Jun-Ho ChoiMinhaeng Cho
Apr 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Reinhard Schweitzer-Stenner, Thomas J Measey
May 2, 2013·The Journal of Physical Chemistry. B·Reinhard Schweitzer-Stenner
Jul 26, 2014·Biomolecules·Siobhan Toal, Reinhard Schweitzer-Stenner
Apr 3, 2007·Journal of Molecular Biology·Nikolaos G SgourakisAngel E Garcia
Oct 16, 2007·Angewandte Chemie·Ian W Hamley
Jun 28, 2011·Journal of Biophotonics·Mingjuan Wang, Renee D Jiji
Jan 24, 2018·The Journal of Physical Chemistry. B·Ian W HamleyDganit Danino
Jun 4, 2019·ACS Applied Bio Materials·Charlotte J C Edwards-GayleJanne Ruokolainen
Jul 31, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Valeria CastellettoJanne Ruokolainen

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