Salmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine

Journal of Medical Microbiology
Bradley L BearsonJ D Kich

Abstract

Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNA-Seq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Vaccination significantly reduced fever and interferon-gamma (IFNγ) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacteri...Continue Reading

References

Nov 15, 2008·Molecular Microbiology·Jörg Vogel
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jan 14, 2010·Journal of Molecular Microbiology and Biotechnology·Miguel Angel De la Cruz, Edmundo Calva
Feb 16, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jun Lu, David G Grenache
Aug 2, 2011·Cytokine & Growth Factor Reviews·Mingli LiuJonathan K Stiles
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Feb 3, 2016·Vaccine·Bradley L BearsonJalusa D Kich
Feb 9, 2016·Zoonoses and Public Health·A D Wales, R H Davies
Mar 24, 2016·Risk Analysis : an Official Publication of the Society for Risk Analysis·Emma L SnaryAndrew A Hill

❮ Previous
Next ❯

Citations

Feb 8, 2018·Foodborne Pathogens and Disease·Daniel C ShippyShawn M D Bearson
Jan 1, 2020·Porcine Health Management·Briony M AtkinsonShawn M D Bearson
Mar 20, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Marta CampilloTanja Opriessnig
Aug 11, 2020·Virology·Hironobu MurakamiKenji Tsukamoto

❮ Previous
Next ❯

Datasets Mentioned

BETA
CP003386.1

Methods Mentioned

BETA
sedation
ELISA
RNA-Seq

Software Mentioned

topGo
GraphPad Prism
DESeq2
Bowtie aligner
Ensembl Sscrofa10
Samtools
CLC Genomics Workbench
GraphPad
Bowtie
FastQC

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.