Salmonella effectors within a single pathogenicity island are differentially expressed and translocated by separate type III secretion systems

Molecular Microbiology
Leigh A KnodlerB Brett Finlay

Abstract

Pathogenicity islands (PAIs) are large DNA segments in the genomes of bacterial pathogens that encode virulence factors. Five PAIs have been identified in the Gram-negative bacterium Salmonella enterica. Two of these PAIs, Salmonella pathogenicity island (SPI)-1 and SPI-2, encode type III secretion systems (TTSS), which are essential virulence determinants. These 'molecular syringes' inject effectors directly into the host cell, whereupon they manipulate host cell functions. These effectors are either encoded with their respective TTSS or scattered elsewhere on the Salmonella chromosome. Importantly, SPI-1 and SPI-2 are expressed under distinct environmental conditions: SPI-1 is induced upon initial contact with the host cell, whereas SPI-2 is induced intracellularly. Here, we demonstrate that a single PAI, in this case SPI-5, can encode effectors that are induced by distinct regulatory cues and targeted to different TTSS. SPI-5 encodes the SPI-1 TTSS translocated effector, SigD/SopB. In contrast, we report that the adjacently encoded effector PipB is part of the SPI-2 regulon. PipB is translocated by the SPI-2 TTSS to the Salmonella-containing vacuole and Salmonella-induced filaments. We also show that regions of SPI-5 are not...Continue Reading

References

Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E Galán, R Curtiss
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·V L Miller, J J Mekalanos
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F Garcia-del PortilloB B Finlay
Jun 1, 1993·Journal of General Microbiology·E F BoydR K Selander
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E SheaD W Holden
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·H OchmanE A Groisman
Sep 1, 1996·Molecular Microbiology·L M ChenJ E Galán
Dec 1, 1996·Infection and Immunity·H Ochman, E A Groisman
Mar 1, 1996·Applied and Environmental Microbiology·E F BoydR K Selander
Mar 1, 1997·Journal of Bacteriology·E F BoydR K Selander
Apr 16, 1998·Journal of Bacteriology·K H Hong, V L Miller
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·C J Hueck
Sep 24, 1998·Infection and Immunity·A J BäumlerL G Adams
Nov 24, 1998·Infection and Immunity·M A JonesE E Galyov
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·F A NorrisP W Majerus
Jan 28, 1999·Journal of Bacteriology·A B Blanc-PotardE A Groisman
Apr 21, 1999·Molecular Microbiology·J DeiwickM Hensel
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·G M YoungV L Miller
Sep 16, 1999·The EMBO Journal·R D Hayward, V Koronakis
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·E A Miao, S I Miller
Sep 9, 2000·The Journal of Biological Chemistry·O Steele-MortimerB B Finlay

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Citations

Jun 20, 2007·Folia Microbiologica·K PapezovaI Rychlik
Apr 6, 2004·Advanced Drug Delivery Reviews·V K ViswanathanGail Hecht
Jun 6, 2003·Microbes and Infection·Brian K CoombesB Brett Finlay
Sep 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Víctor H BustamanteJosé L Puente
Nov 26, 2009·Vector Borne and Zoonotic Diseases·Andreia M LefferAntonio J Piantino Ferreira
Jul 1, 2005·Molecular Biology of the Cell·Leigh A Knodler, Olivia Steele-Mortimer
Dec 25, 2004·Cellular Microbiology·Dan DrecktrahOlivia Steele-Mortimer
Jan 17, 2004·Clinical Microbiology Reviews·Herbert Schmidt, Michael Hensel
May 11, 2011·Infection and Immunity·Winnie W S KumB Brett Finlay
Apr 23, 2004·Infection and Immunity·Imke Hansen-WesterMichael Hensel
Aug 24, 2004·Infection and Immunity·Sonya L Kujat ChoyB Brett Finlay
Jan 24, 2006·Infection and Immunity·Sofia Eriksson YgbergMikael Rhen
Feb 20, 2007·Journal of Bacteriology·Umaporn SilphaduangBrian K Coombes
Mar 30, 2011·PloS One·Nat F BrownB Brett Finlay
Nov 24, 2005·PLoS Pathogens·Nat F BrownB Brett Finlay
Aug 31, 2011·PLoS Pathogens·Maria FookesNicholas R Thomson
Jan 30, 2013·PLoS Pathogens·Stacey D GilkRobert A Heinzen
Nov 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michelle L ZaharikB Brett Finlay
Sep 5, 2014·Foodborne Pathogens and Disease·Guojie CaoJianghong Meng
Jul 26, 2011·International Journal of Medical Microbiology : IJMM·Claudio Vieira da SilvaBellisa de Freitas Barbosa
Mar 7, 2009·Current Opinion in Microbiology·Ephraim Fass, Eduardo A Groisman
Feb 29, 2008·Current Opinion in Microbiology·Olivia Steele-Mortimer
Nov 4, 2004·Molecular Microbiology·Eirwen MorganTimothy S Wallis
Jul 17, 2003·Cellular Microbiology·Scott R Waterman, David W Holden

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