Salmonella flagellin is a potent carrier-adjuvant for peptide conjugate to induce peptide-specific antibody response in mice

Vaccine
Feng QianHu-Ji Xu

Abstract

As an agonist to innate immune system, Salmonella flagellin has been proven to be a potent adjuvant either admixed or genetically fused with antigens and applied to a variety of vaccines against infectious diseases. However, relatively little is known about its carrier-adjuvant effect for conjugate vaccine. Conjugation is an effective approach often used to make haptens such as some peptides and polysaccharides immunogenic and in some cases used to make poor immunogens more immunogenic. In the current study, Salmonella flagellin was tested for its carrier-adjuvant effect in a peptide conjugation. The recombinant Salmonella flagellin (rFliC) purified from Escherichia coli was firstly modified by maleimide groups, then coupled with a synthetic peptide (EXP153:CDNNLVSGP) that is a B-cell epitope derived from Plasmodium falciparum exported protein-1 to generate the conjugate of EXP153-rFliC. Bioactivity assay showed that both chemical modification and conjugation did not apparently impair the TLR5-ligand activity of rFliC. EXP153-rFliC was used to immunize BALB/c mice via subcutaneous route, and the sera obtained from immunized mice were examined by ELISA and IFA. While no detectable antibody responses were induced by the peptide a...Continue Reading

References

Oct 3, 2003·The New England Journal of Medicine·Ngoc Lanh MaiShousun C Szu
Sep 24, 2004·European Journal of Immunology·Adam F CunninghamIan C M MacLennan
Jan 20, 2006·Molecular Microbiology·Tobias SpielmannDavid J Kemp
Nov 15, 2006·Current Pharmaceutical Design·Ken J IshiiShizuo Akira
Nov 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yimin WuLouis H Miller
Dec 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Joanna Kubler-KielbRachel Schneerson
Mar 21, 2007·Journal of Clinical Immunology·Ken J Ishii, Shizuo Akira
May 2, 2007·The Journal of Infectious Diseases·William F McDonaldT J Powell
Aug 11, 2007·Seminars in Immunopathology·Edward A MiaoAlan Aderem
Nov 7, 2008·Clinical and Vaccine Immunology : CVI·Steven B MizelBrian Bell
Aug 1, 2009·Malaria Journal·Ipsita Pal BhowmickGotam K Jarori
Oct 6, 2009·The Journal of Biological Chemistry·Zhenyu CaiDekai Zhang
Nov 13, 2010·European Journal of Immunology·Matam Vijay-KumarAndrew T Gewirtz
Dec 20, 2011·Methods : a Companion to Methods in Enzymology·N H TrierG Houen
Mar 24, 2012·Current Opinion in Virology·Taiki AoshiKen J Ishii
Apr 24, 2012·Current Opinion in Immunology·Carl R AlvingSteven G Reed
Jul 31, 2012·Therapeutic Delivery·Yoshio Fujita, Hiroaki Taguchi
Oct 26, 2012·Human Vaccines & Immunotherapeutics·Philippe Moingeon
Oct 30, 2012·Cell·Ramkumar MathurSankar Ghosh

❮ Previous
Next ❯

Citations

Feb 9, 2016·Zoonoses and Public Health·A D Wales, R H Davies
Mar 10, 2017·Expert Review of Vaccines·Brandon SackD Noah Sather
Aug 25, 2020·Journal of Biomedical Science·Tzu-Wei ChiuYuan Chuan Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.