Salmonella-Mediated Inflammation Eliminates Competitors for Fructose-Asparagine in the Gut.

Infection and Immunity
Jikang WuBrian M M Ahmer

Abstract

Salmonella enterica elicits intestinal inflammation to gain access to nutrients. One of these nutrients is fructose-asparagine (F-Asn). The availability of F-Asn to Salmonella during infection is dependent upon Salmonella pathogenicity islands 1 and 2, which in turn are required to provoke inflammation. Here, we determined that F-Asn is present in mouse chow at approximately 400 pmol/mg (dry weight). F-Asn is also present in the intestinal tract of germfree mice at 2,700 pmol/mg (dry weight) and in the intestinal tract of conventional mice at 9 to 28 pmol/mg. These findings suggest that the mouse intestinal microbiota consumes F-Asn. We utilized heavy-labeled precursors of F-Asn to monitor its formation in the intestine, in the presence or absence of inflammation, and none was observed. Finally, we determined that some members of the class Clostridia encode F-Asn utilization pathways and that they are eliminated from highly inflamed Salmonella-infected mice. Collectively, our studies identify the source of F-Asn as the diet and that Salmonella-mediated inflammation is required to eliminate competitors and allow the pathogen nearly exclusive access to this nutrient.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 1, 1954·Proceedings of the Society for Experimental Biology and Medicine·M BOHNHOFFC P MILLER
Sep 1, 1962·The Journal of Infectious Diseases·M BOHNHOFF, C P MILLER
Sep 6, 2005·Trends in Microbiology·Siegfried Hapfelmeier, Wolf-Dietrich Hardt
Jan 20, 2009·Bioinformatics·Andrew M WaterhouseGeoffrey J Barton
May 22, 2009·Gastroenterology·Jean C Buzby, Tanya Roberts
Feb 18, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shannon E MajowiczUNKNOWN International Collaboration on Enteric Disease 'Burden of Illness' Studies
Sep 15, 2010·Advances in Carbohydrate Chemistry and Biochemistry·Valeri V Mossine, Thomas P Mawhinney
Sep 25, 2010·Nature·Sebastian E WinterAndreas J Bäumler
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Parameth ThiennimitrAndreas J Bäumler
Jan 22, 2013·Gut Microbes·Ana Victoria C PilarBrian K Coombes
Mar 12, 2013·Nature Reviews. Microbiology·Bärbel StecherWolf-Dietrich Hardt
May 3, 2013·PLoS Pathogens·Fabian Rivera-ChávezAndreas J Bäumler
May 8, 2014·Immunology Letters·Franziska Faber, Andreas J Bäumler
May 24, 2015·Annual Review of Microbiology·Fabian Rivera-Chávez, Andreas J Bäumler
Jun 4, 2016·Carbohydrate Research·Alexandar L Hansen, Edward J Behrman
Jun 17, 2016·Nature·Franziska FaberAndreas J Bäumler
Jul 8, 2016·Nature·Andreas J Bäumler, Vanessa Sperandio
Sep 7, 2016·Nature Microbiology·Rebecca A DalyKelly C Wrighton
Dec 14, 2016·The ISME Journal·Lindsey M SoldenKelly C Wrighton
Jan 6, 2017·PLoS Pathogens·Franziska FaberAndreas J Bäumler
Jan 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Aaron M NussPetra Dersch
Feb 9, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Pradip K BiswasVenkat Gopalan
Apr 30, 2017·Microbiome·Mikayla A BortonKelly C Wrighton
Aug 30, 2017·Journal of Bacteriology·Anice Sabag-DaigleBrian M M Ahmer
Dec 13, 2017·Journal of Agricultural and Food Chemistry·Jikang WuBrian M M Ahmer
Dec 23, 2017·Applied and Environmental Microbiology·Anice Sabag-DaigleBrian M M Ahmer
Dec 26, 2017·Cell Host & Microbe·Caroline C GillisSebastian E Winter

❮ Previous
Next ❯

Citations

May 24, 2018·FEMS Microbiology Reviews·Hirotaka HiyoshiAndreas J Bäumler
Aug 9, 2020·Nucleic Acids Research·Michael ShafferKelly C Wrighton
Feb 27, 2020·Journal of Animal Science and Biotechnology·Pingting GuoPingli He
Nov 11, 2020·Journal of Bacteriology·Robert W P Glowacki, Eric C Martens
Sep 30, 2021·Microbiology and Molecular Biology Reviews : MMBR·Erin F BoulangerBrian M M Ahmer

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.