Salmonella spp. transmission in a vertically integrated poultry operation: Clustering and diversity analysis using phenotyping (serotyping, phage typing) and genotyping (MLVA)

PloS One
Helen Kathleen CrabbJames Rudkin Gilkerson

Abstract

The transmission of Salmonella enterica within a vertically integrated poultry operation was investigated longitudinally over an 18-month period (2013-2014). Thirty six percent of all samples collected (1503 of 4219) were positive for salmonellae with seven Salmonella enterica subsp. enterica serovars, and one Salmonella enterica subsp. salamae serovar detected. Both Salmonella enterica subsp. enterica serovars Infantis and Typhimurium were detected in all locations sampled. Salmonella Typhimurium was the most frequently detected serovar (63% of serotyped samples) with 8 phage types (PT) and 41 multiple-locus variable-number tandem-repeats analysis (MLVA) profiles identified. The most frequently identified phage types were PT135a and DT135. A total of 62 PT/MLVA combinations were observed. MLVA profiles 03-14-10-09-525 and 03-15-11-11-525 were the most frequently identified and 83% of the isolates shared at least one MLVA profile with an isolate from another phage type. The use of phage typing and MLVA profiling, on their own or in combination, were insufficient to understand the complexity of the epidemiological relationships between locations within this production system. Despite the high level of apparent diversity, cluster...Continue Reading

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Citations

May 6, 2019·Applied and Environmental Microbiology·Helen K CrabbJames Rudkin Gilkerson
Feb 19, 2019·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (EFSA BIOHAZ Panel)Robert Davies
Aug 23, 2020·Animals : an Open Access Journal From MDPI·Sandra Sevilla-NavarroClara Marin
Feb 18, 2021·Zoonoses and Public Health·Johana Elizabeth DominguezPablo Anibal Chacana
May 11, 2021·Frontiers in Medicine·Nitya SinghArie H Havelaar
Jul 1, 2021·Veterinary Research Communications·Shafayat ZamilHimel Barua

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Methods Mentioned

BETA
PCR
Serotyping
genotyping

Software Mentioned

pvcluster
BiodiversityR
factoextra
Phyloviz
R
VennDiagram
FactoMineR
vegan

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