Salmonella strains and mammalian cells genetically engineered for expression of sulfotransferases

Toxicology Letters
H GlattC N Falany

Abstract

Rat and human sulfotransferases (STs) were expressed in his- S. typhimurium strains. These new bacterial strains detected various mutagens which are difficult to recognize in traditional test systems, including benzylic alcohols derived from polycyclic aromatic hydrocarbons, hycanthone and 1'-hydroxysafrole. STs were also stably expressed in V79 Chinese hamster cells, which do not express endogenous ST and are suitable for the detection of genotoxic effects. Positive responses in these test systems were observed with various benzylic alcohols, including benzo[a]pyrene-7,8,9,10-tetrols. We demonstrate that a few reactive sulfuric acid conjugates are efficiently detected as genotoxicants only when generated directly within the indicator cell.

References

Jul 1, 1991·Trends in Pharmacological Sciences·C N Falany
Jun 1, 1994·Chemico-biological Interactions·J A Miller
Jan 1, 1993·European Journal of Clinical Pharmacology·G M PacificiL Giuliani
Mar 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·C N FalanyJ L Falany

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Citations

Aug 1, 1998·Mutation Research·F P Guengerich, T Shimada
Nov 14, 1997·Current Opinion in Biotechnology·F P Guengerich, A Parikh
Nov 11, 2011·Environmental and Molecular Mutagenesis·Yoshimitsu OdaMin Yang

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