Salmonella typhimurium metC operator-constitutive mutations

FEMS Microbiology Letters
Y M Park, G Stauffer

Abstract

We used an Escherichia coli lac deletion strain lysogenized with a metC-lacZ fusion phage (lambda Clac) to select operator-constitutive mutations in the Salmonella typhimurium metC gene control region. The mutations were located in a region containing 2 tandemly repeated 8 bp palindromes previously proposed to be the MetJ repressor binding site. Lysogens carrying lambda Clac mutant phage exhibit high beta-galactosidase levels that are only partially repressible by methionine. The results suggest that the mutations disrupt the methionine control system mediated by the metJ gene product.

Citations

Aug 1, 2020·JMIR Research Protocols·Bastiaan Johannes Paulus Cornelis WillemseFleur Hasaart

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