PMID: 11934440Apr 6, 2002Paper

Salmonella typhimurium mutagenicity tester strains that overexpress oxygen-insensitive nitroreductases nfsA and nfsB

Mutation Research
C C CarrollI B Lambert

Abstract

We have designed and constructed a series of plasmids that contain the major and/or minor Escherichia coli nitroreductase genes, nfsA and nfsB, in different combinations with R plasmid mucA/B genes and the Salmonella typhimurium OAT gene. The plasmid encoded gene products are necessary for both the metabolic activation of a range of structurally diverse nitrosubstituted compounds, and for mutagenic translation bypass. Introduction of these plasmids into S. typhimurium TA1538 and TA1535 has created several new tester strains which exhibit an extremely high mutagenic sensitivity and a broad substrate specificity towards a battery of nitrosubstituted test compounds that included 4-nitroquinoline-1-oxide (4-NQO), nitrofurazone (NF), 1-nitropyrene (1-NP), 2-nitronaphthalene (2-NN), 2-nitrofluorene (2-NF), and 1,6-dinitropyrene (1,6-DNP). Our studies show that the nfsA gene encodes a product that is extremely effective in the metabolic activation of a range of structurally diverse nitrosubstituted compounds. Several of the new tester strains are more than two orders of magnitude more sensitive to nitrosubstituted compounds than the Ames tester strains TA100 or TA98. In addition to enhancing mutagenic sensitivity, plasmids encoding bo...Continue Reading

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Citations

Oct 19, 2004·Applied Microbiology and Biotechnology·Hong YinBarth F Smets
Sep 10, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Marco Vinicio Ramírez-Mares, Elvira González de Mejía
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May 12, 2009·Mutation Research·Javier Espinosa-AguirreRafael Camacho-Carranza
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Aug 26, 2010·Environmental Toxicology·Aurélie BonnefoyAlain Botta
May 27, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Sandra Martínez-PucholJoaquim Ruiz
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Dec 21, 2004·Chemical Research in Toxicology·Mariko MurataShosuke Kawanishi
Oct 7, 2021·Applied and Environmental Microbiology·Yao ZhouShengmin Zhou

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