Salmonella typhimurium mutants of RfaH-phenotype: genetics and antibiotic sensitivities

Journal of General Microbiology
B A StockerW Takano

Abstract

Transductional mapping, with phage ES18 or ES18.hl, showed that several mutations causing the RfaH- phenotype (defective formation of galactose I and also of more distal units of the lipopolysaccharide core) were located between metE and pepQ in the Salmonella typhimurium linkage map; the affected locus is designated rfaH. The mutation of one strain of RfaH- phenotype was located elsewhere, at an unidentified rfa locus. Introduction of an F' plasmid containing the metE segment of the Escherichia coli chromosome into several rfaH mutants restored the "smooth" (Rfa+) phenotype. Several rfaH mutations, and that of the phenotypically similar rfa mutant, caused increased sensitivity to bacitracin, polymyxin, novobiocin, nafcillin and oxacillin, as expected if the mutations have no effect on the formation of the part of the lipopolysaccharide core proximal to the galactose units.

Citations

Nov 20, 1997·Transplantation Proceedings·H KajiM Imamura
Sep 1, 1996·Progress in Lipid Research·S G Wilkinson
Jul 9, 1999·Progress in Lipid Research·C C DiRussoJ D Weimar
Jul 5, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·I N Okeke, R Edelman
Sep 4, 2007·Journal of the American Veterinary Medical Association·Douglas R MaderDorr G Dearborn
Oct 15, 2009·Future Microbiology·Michel R Popoff, Philippe Bouvet
Apr 17, 2014·The Journal of Organic Chemistry·Rubén TapiaEnrique Alvarez-Manzaneda

Related Concepts

Antibiotics
Genome Mapping
Crosses, Genetic
Fungus Drug Sensitivity Tests
Salmonella Phages
Salmonella typhimurium LT2
Selection, Genetic
Transduction, Genetic

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