PMID: 8584789Oct 1, 1995Paper

Salmonella typhimurium TnphoA mutants with increased sensitivity to biological and chemical detergents

Research in Microbiology
F J LacroixP Pardon

Abstract

Salmonella typhimurium is a ubiquitous pathogenic bacterium able to sustain the environmental conditions of the gastrointestinal tract, including biliary salts. To understand the mechanisms involved in bile salt resistance and, more generally, detergent resistance, we investigated S. typhimurium mutants produced with the random mutagenic TnphoA transposon. A total of 3,000 transpositional mutants were isolated. Three strains among the 1,432 first mutants lost the ability to grow in the presence of biological and chemical detergents. They were prototrophic and exhibited normal lipopolysaccharide and outer membrane protein profiles after SDS-PAGE. They did not show sensitivity to dyes but showed very different sensitivities to antibiotics. For each mutant strain, Southern blotting analysis revealed a unique TnphoA insertion at different chromosomal locations. These observations were confirmed by transduction experiments.

References

Feb 27, 1976·Molecular & General Genetics : MGG·D N Antón, L V Orce
Feb 1, 1975·The American Journal of Clinical Nutrition·H J BinderM Floch
Jan 1, 1977·Annual Review of Microbiology·D C Savage
Apr 1, 1992·Molecular Microbiology·K W Nickerson, A Aspedon
Nov 1, 1991·Molecular & General Genetics : MGG·J R KleinR Plapp
Jul 1, 1985·Journal of Bacteriology·S K Hoiseth, B A Stocker
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C Manoil, J Beckwith
Dec 1, 1988·Microbiological Reviews·K E Sanderson, J R Roth
Jan 1, 1987·Annales De L'Institut Pasteur. Microbiology·A J Winter
Jul 1, 1986·Annales De L'Institut Pasteur. Microbiology·P PardonI Miras
Oct 1, 1967·Journal of Bacteriology·R Nagel de Zwaig, S E Luria
May 1, 1972·Journal of General Microbiology·R G WilkinsonB A Stocker
Jan 1, 1984·Annual Review of Microbiology·R E Hancock
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Dec 1, 1993·Microbiological Reviews·M Riley
Nov 1, 1993·Antimicrobial Agents and Chemotherapy·M Vaara
Jun 1, 1993·Applied and Environmental Microbiology·G J Leyer, E A Johnson
Aug 1, 1956·Journal of General Microbiology·G FURNESS, D ROWLEY

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Citations

May 15, 2002·FEMS Microbiology Reviews·Laura J V Piddock
Aug 17, 2005·FEMS Microbiology Reviews·Máire BegleyColin Hill
Mar 30, 2017·Infection and Immunity·Kourtney P NickersonChristina S Faherty
Mar 13, 2010·Science Progress·Petra Louis, Conor P O'Byrne

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