Salmonella virulence effector SopE and Host GEF ARNO cooperate to recruit and activate WAVE to trigger bacterial invasion.

Cell Host & Microbe
Daniel HumphreysVassilis Koronakis

Abstract

Salmonella virulence effectors elicit host cell membrane ruffling to facilitate pathogen invasion. The WAVE regulatory complex (WRC) governs the underlying membrane-localized actin polymerization, but how Salmonella manipulates WRC is unknown. We show that Rho GTPase activation by the Salmonella guanine nucleotide exchange factor (GEF) SopE efficiently triggered WRC recruitment but not its activation, which required host Arf GTPase activity. Invading Salmonella recruited and activated Arf1 to facilitate ruffling and uptake. Arf3 and Arf6 could also enhance invasion. RNAi screening of host Arf-family GEFs revealed a key role for ARNO in pathogen invasion and generation of pathogen-containing macropinosomes enriched in Arf1 and WRC. Salmonella recruited ARNO via Arf6 and the phosphoinositide phosphatase effector SopB-induced PIP3 generation. ARNO in turn triggered WRC recruitment and activation, which was dramatically enhanced when SopE and ARNO cooperated. Thus, we uncover a mechanism by which pathogen and host GEFs synergize to regulate WRC and trigger Salmonella invasion.

References

Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·E MeacciM Vaughan
Feb 7, 1998·The Journal of Biological Chemistry·S FrankJ E Casanova
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MacheskyT D Pollard
Jan 22, 2000·The Biochemical Journal·K Venkateswarlu, P J Cullen
Sep 20, 2000·The Journal of Cell Biology·R RohatgiM W Kirschner
Jul 7, 2001·The Journal of Biological Chemistry·A FriebelW D Hardt
Nov 1, 2001·Annual Review of Cell and Developmental Biology·J E Galán
Mar 3, 2004·Molecular Cell·Emma J McGhieVassilis Koronakis
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alexis GautreauMarc W Kirschner
Jun 3, 2005·The Journal of Biological Chemistry·Jing ShiJames E Casanova
Jan 18, 2006·Methods in Enzymology·Hye-Young YoonPaul A Randazzo
Jan 18, 2006·Methods in Enzymology·Kazuhisa Nakayama, Hiroyuki Takatsu
Nov 1, 2006·The Journal of Cell Biology·Jayesh C Patel, Jorge E Galán
Dec 15, 2006·Nature·Markus HafnerMichael Famulok
Apr 6, 2007·Molecular Biology of the Cell·Lee Ann CohenJulie G Donaldson
Jan 23, 2009·Current Opinion in Microbiology·Emma J McGhieVassilis Koronakis
Sep 8, 2009·Cellular Microbiology·Jan HänischKlemens Rottner
Nov 18, 2009·Molecular Cell·Andres M Lebensohn, Marc W Kirschner
Mar 20, 2010·Nature Reviews. Molecular Cell Biology·Kenneth G Campellone, Matthew D Welch
Nov 26, 2010·Nature·Zhucheng ChenMichael K Rosen
Jan 25, 2011·Current Biology : CB·Andrew J Davidson, Robert H Insall
Mar 17, 2011·Molecular Systems Biology·Benjamin MisselwitzWolf-Dietrich Hardt
May 19, 2011·Nature Reviews. Molecular Cell Biology·Julie G Donaldson, Catherine L Jackson
Aug 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Vassilis KoronakisEmma J McGhie

❮ Previous
Next ❯

Citations

Apr 2, 2013·Research in Microbiology·Tommaso TosiAndréa Dessen
Oct 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Daniel HumphreysVassilis Koronakis
Oct 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sun-Young JeongStanley N Cohen
Feb 6, 2013·Current Opinion in Gastroenterology·Kim Hodges, Gail Hecht
Sep 21, 2012·Journal of Cell Science·Daniel HumphreysVassilis Koronakis
Jun 3, 2014·BMC Genomics·Nathan L BachmannScott A Beatson
Jan 30, 2013·Critical Reviews in Microbiology·Mehari Tesfazgi MebrhatuAbram Aertsen
May 21, 2013·FEBS Letters·Danièle Stalder, Bruno Antonny
Jan 16, 2013·Current Opinion in Microbiology·Nathalie Carayol, Guy Tran Van Nhieu
Dec 3, 2014·Cellular Microbiology·Marcela de Souza Santos, Kim Orth
Dec 4, 2014·Cellular Microbiology·Cesar M Valencia-GallardoGuy Tran Van Nhieu
Apr 25, 2013·Virulence·Louis-Charles Fortier, Ognjen Sekulovic
Oct 23, 2012·Cell Host & Microbe·Helen Sarantis, Sergio Grinstein
Mar 10, 2015·Nature Reviews. Microbiology·Doris L LaRockSamuel I Miller
Feb 18, 2015·Frontiers in Cellular and Infection Microbiology·Thomas DandekarJanina Noster
Dec 23, 2016·The Journal of Cell Biology·Nisha Bte Mohd RafiqAlexander D Bershadsky
May 20, 2017·Small GTPases·Vikash SinghVassilis Koronakis
Aug 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Monica I AlvarezDennis C Ko
May 5, 2020·PLoS Pathogens·Stefan A FattingerMikael E Sellin
May 1, 2020·Cellular Microbiology·Carolina Varela-ChavezMichel R Popoff
Jun 28, 2013·The Journal of Biological Chemistry·Manuel WoltersMartin Aepfelbacher
Mar 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Andrew B E BrooksVassilis Koronakis
Nov 9, 2017·Molecular Microbiology·Patrick J Woida, Karla J F Satchell
Aug 21, 2019·Frontiers in Cellular and Infection Microbiology·Lixin LouYang Wang
Aug 30, 2017·Frontiers in Cellular and Infection Microbiology·Peter J HumeVassilis Koronakis
Jan 24, 2019·International Journal of Molecular Sciences·Christine E TannaPei-Wen Chen
Dec 28, 2019·International Journal of Molecular Sciences·Liam Caven, Rey A Carabeo
Apr 8, 2020·International Journal of Molecular Sciences·Vikash SinghVassilis Koronakis
May 6, 2021·Proceedings of the National Academy of Sciences of the United States of America·Marcel HahnIvan Dikic
Jun 4, 2019·Microbiology Spectrum·Marcela De Souza Santos, Kim Orth
Jul 10, 2021·PLoS Pathogens·Jeffrey S BourgeoisDennis C Ko

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPases
GTPase
transfection
fluorescence microscopy
nucleotide exchange
Assay

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Daniel HumphreysVassilis Koronakis
Proceedings of the National Academy of Sciences of the United States of America
Vassilis KoronakisEmma J McGhie
Cellular Microbiology
Robert C Orchard, Neal M Alto
© 2022 Meta ULC. All rights reserved