Abstract
Despite extensive efforts to study the inflammatory process in the central nervous system of Parkinson's disease (PD) patients, little is known about the role of peripheral blood mononuclear cells (PBMCs) in PD. In the present study, we used an in vitro co-culture system to study the role of the human monocyte cell line THP-1 in medium conditioned by the neuroblastoma cell line SH-SY5Y damaged with the endogenous neurotoxin 1-methyl-4-phenyl-1,2,3,4-tetrahydroisoquinoline (Salsolinol, Sal) in co-culture with the human glioma cell line U87. For this purpose, SH-SY5Y and U87 co-cultures were treated with Sal, and this conditioned medium containing mediators, including the potential effector CCL2, was isolated and applied to THP-1 cells. This treatment resulted in approximately 19 % cell proliferation as well as activation of mTOR and induction of phosphorylated 4E-BP1, S6K1, PI3K, and AKT proteins. Treatment with rapamycin, an mTOR inhibitor, attenuated the proliferation of THP-1 cells. U87 glial cells were essential for this as medium conditioned without them had no effect on THP-1 cells. These results suggest a positive effect of THP-1 cells on Sal-induced neurotoxicity in a cellular model of PD and this is likely mediated by t...Continue Reading
References
Dec 1, 1979·Psychiatry Research·G C DavisI J Kopin
Jan 1, 1988·Acta Neuropathologica·P L McGeerE G McGeer
Jul 1, 1985·Neurology·P A BallardJ W Langston
Oct 24, 1994·Neuroscience Letters·M MogiT Nagatsu
Dec 29, 1995·Neuroscience Letters·D Blum-DegenP Riederer
Jan 29, 1999·Archives of Neurology·D J GelbS Gilman
Jan 3, 2001·Kidney International·R N SaundersM L Nicholson
Aug 30, 2002·Neurotoxicology and Teratology·Makoto NaoiHong Yi
May 20, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M Merle EllosoSteven J Adelman
Jun 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·David R ParkW Conrad Liles
Dec 31, 2003·Neurotoxicology·Makoto NaoiGeorgy M Nagy
Mar 23, 2004·Annals of the New York Academy of Sciences·Cristina ColomboGianmario Frigo
Jun 3, 2004·Lancet·Ali SamiiBruce R Ransom
Feb 19, 2005·Science·D D SarbassovDavid M Sabatini
Oct 14, 2005·The Journal of Biological Chemistry·Richard C Hresko, Mike Mueckler
Mar 28, 2006·Neurobiology of Disease·Alessandro PrigioneCarlo Ferrarese
Apr 11, 2006·Molecular Cell·Dos D SarbassovDavid M Sabatini
Aug 8, 2006·Brain : a Journal of Neurology·Marija DjukicMarco Prinz
Feb 13, 2007·Journal of the American College of Cardiology·Stefan VerheyeGuido R Y De Meyer
Mar 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Matthew P MountDavid S Park
Mar 20, 2009·Human Molecular Genetics·Suzanne Lesage, Alexis Brice
Aug 25, 2009·Oncogene·P KalerL Klampfer
May 12, 2010·Current Alzheimer Research·C UllrichC Humpel
Nov 24, 2011·Neuroscience·J Bové, C Perier
Nov 30, 2011·Methods in Molecular Biology·Thomas Weichhart
Aug 9, 2012·Journal of Neuroinflammation·Valerio PisaniAntonio Pisani
Aug 23, 2012·Cytokine·Dariusz KoziorowskiAndrzej Friedman
Nov 3, 2012·Neurochemical Research·Kangyong LiuXiaojiang Sun
Feb 13, 2013·Movement Disorders : Official Journal of the Movement Disorder Society·Natalja FunkSaskia Biskup
May 15, 2013·Cellular and Molecular Life Sciences : CMLS·Michela Deleidi, Thomas Gasser
Jun 14, 2013·Immunology·Sandra Amor, M Nicola Woodroofe
Jun 15, 2013·Cell Death & Disease·M G ThomasR B Parsons
Sep 24, 2013·PloS One·Shyra J GardaiJennifer A Johnston
Nov 28, 2013·Journal of Parkinson's Disease·George T KannarkatMalú G Tansey
Jun 17, 2014·International Journal of Molecular Sciences·Fuli WangYulin Deng
Oct 7, 2014·Acta Neuropathologica·Veselin GrozdanovKarin M Danzer