PMID: 7023537Jun 23, 1981Paper

Salt dependence of the kinetics of the lac repressor-operator interaction: role of nonoperator deoxyribonucleic acid in the association reaction

Biochemistry
M D Barkley

Abstract

The kinetics of binding of lac repressor protein and operator deoxyribonucleic acid (DNA) have been studied as a function of monovalent and divalent cation concentration. The salt dependence of the association and dissociation rate constants has been interpreted in light of recent theoretical analyses based on Manning's counterion condensation model. The bell-shaped dependence of the association rate constant on salt concentration evidences a role for nonoperator DNA binding in the repressor's search for the operator site on a large DNA molecule. At intermediate mono- or divalent cation concentrations, the association rate goes through a maximum. At lower cation concentrations, it decreases and becomes dependent on DNA concentration; the high affinity of repressor for nonoperator DNA confines the protein to the DNA. At higher cation concentrations, the association rate decreases and becomes dependent on the weak affinity of repressor for nonoperator DNA. The kinetic data are fit to the theory of Berg and Blomberg [Berg, O. G., & Blomberg, C. (1978) Biophys. Chem. 8, 271] for the salt dependence of association kinetics with coupled diffusion, using published values of the affinity for nonoperator DNA. From this fit, one-dimensio...Continue Reading

References

Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·Z E KahanaB F Erlanger
Sep 27, 1978·Biochimica Et Biophysica Acta·M Pfahl
Jan 1, 1978·Biophysical Chemistry·C F AndersonP A Hart
Nov 4, 1976·Journal of Molecular Biology·M T RecordP De Haseth
May 1, 1975·Developmental Psychobiology·G W Meier
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·W S ReznikoffC K Hurley
Sep 20, 1974·Nature·J C WangS Bourgeois
Apr 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·B Müller-HillW Gilbert
Feb 28, 1970·Journal of Molecular Biology·A D RiggsS Bourgeois
Nov 14, 1970·Journal of Molecular Biology·A D RiggsM Cohn

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Citations

Jul 5, 1987·Journal of Molecular Biology·J G KimW F Anderson
Sep 10, 1990·Biochimica Et Biophysica Acta·A M KhouryP Lu
Jun 9, 2005·Mutation Research·Dmitry O Zharkov, Arthur P Grollman
Jan 25, 1984·Journal of Molecular Biology·M G Fried, D M Crothers
Nov 11, 1996·Biochimica Et Biophysica Acta·M A FarwellL L Spremulli
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W E JackP Modrich
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·I Wong, T M Lohman
Nov 26, 1984·Nucleic Acids Research·I T Weber, T A Steitz
Jun 5, 2004·Nucleic Acids Research·Stephen E Halford, John F Marko
Jan 26, 2006·Nucleic Acids Research·Robert J FisherAlan Rein
Oct 12, 2010·Nucleic Acids Research·Hsin-Yi YehBan-Yang Chang
Oct 15, 2013·Nucleic Acids Research·Marcin TabakaRobert Hołyst
May 15, 1997·European Journal of Biochemistry·A Pingoud, A Jeltsch
May 5, 2007·Annual Review of Biophysics and Biomolecular Structure·Peter H von Hippel
Jun 5, 1987·Journal of Molecular Biology·K J Martin, R F Schleif
Jun 3, 2010·Angewandte Chemie·Siowling SohBartosz A Grzybowski
Jun 20, 2006·Biophysical Chemistry·Douglas F Stickle, Michael G Fried
Nov 30, 2010·Physical Chemistry Chemical Physics : PCCP·Anatoly B Kolomeisky
Jun 23, 1981·Biochemistry·M D BarkleyG E Sullivan
Jul 1, 1986·Biochemistry·P A WhitsonK S Matthews

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