Salt-dependent studies of NADP-dependent isocitrate dehydrogenase from the halophilic archaeon Haloferax volcanii

Extremophiles : Life Under Extreme Conditions
Dominique MadernGiuseppe Zaccai

Abstract

The salt-dependent stability of recombinant dimeric isocitrate dehydrogenase [ICDH; isocitrate: NADP oxidoreductase (decarboxylating), EC 1.1.1.42] from the halophilic archaeon Haloferax volcanii (Hv) was investigated in various conditions. Hv ICDH dissociation/deactivation was measured to probe the respective effect of anions and cations on stability. Surprisingly, enzyme stability was found to be mainly sensitive to cations and very little (or not) sensitive to anions. Divalent cations induced a strong shift of the active/inactive transition towards low salt concentration. A high resistance of Hv ICDH to chemical denaturation was also found. The data were analysed and are discussed in the framework of the solvation stability model for halophilic proteins.

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Citations

Oct 4, 2005·Extremophiles : Life Under Extreme Conditions·Susana DíazMaría-José Bonete
Feb 21, 2007·Extremophiles : Life Under Extreme Conditions·Soundararajan SrimathiParanji R Narayanan
Jan 15, 2008·Extremophiles : Life Under Extreme Conditions·Yi CaoMin Wu
Aug 27, 2015·Marine Drugs·Montserrat Rodrigo-BañosRosa María Martínez-Espinosa
Mar 5, 2014·Biochimica Et Biophysica Acta·Giuseppe Graziano, Antonello Merlino
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Masateru TakahashiSamir M Hamdan
Jul 23, 2013·HSS Journal : the Musculoskeletal Journal of Hospital for Special Surgery·Anna-Christina BevelaquaScott A Rodeo
Sep 11, 2019·Marine Drugs·Micaela GianiRosa María Martínez-Espinosa
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alka RaniAntonio Zuorro
Apr 7, 2012·Aquatic Biosystems·Ram KaranShiladitya Dassarma

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