Salt-Mediated Nanopore Detection of ADAM-17

ACS Applied Bio Materials
Xiaohan ChenXiyun Guan

Abstract

ADAM-17 (a disintegrin and metalloproteinase 17) plays an important role in various physiological and pathophysiological processes. Overexpression/underexpression of ADAM-17 could lead to various diseases. In this work, by taking advantage of ionic strength and salt gradient, and monitoring the cleavage of a substrate peptide by ADAM-17 in a nanopore, we developed a label-free sensor for the rapid detection of ADAM-17. The sensor was highly sensitive and selective: picomolar concentrations of ADAM-17 could be detected within minutes, while structure similar proteases such as ADAM-9 and MMP-9 did not interfere with its detection. Our developed nanopore sensing strategy should find useful applications in the development of nanopore sensors for other proteases of biological, pharmaceutical, and medical importance.

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Citations

Nov 7, 2019·The Analyst·Golbarg Mohammadi RoozbahaniXiyun Guan
Dec 2, 2020·Angewandte Chemie·Zheng-Li HuYi-Tao Long
Jul 29, 2021·Analytical Chemistry·Xiaohan ChenXiyun Guan
Mar 18, 2020·Analytical Chemistry·Si-Min LuYi-Tao Long

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