Salt mine microorganisms used for the biotransformation of chlorolactones

PloS One
Wanda MączkaMarek Durajczyk

Abstract

The aim of the project was to find new catalysts capable of chlorolactone biotransformation. Three bicyclic chlorolactones with structures possessing one or two methyl groups in their cyclohexane ring were subjected to screening biotransformation using seven bacterial strains and one fungal strain from a salt mine. Three strains of bacteria (Micrococcus luteus Pb10, Micrococcus luteus WSP45, Gordonia alkanivorans Pd25) and one fungal strain (Aspergillus sydowii KGJ10) were able to catalyse hydrolytic dehalogenation of one substrate. The classification of the strains that were effective biocatalysts was confirmed by 16S rDNA analysis. The best result (76%) was obtained using Aspergillus sydowii KGJ10. All strains catalysed hydrolytic dehalogenation without changing the conformation. The equatorial position of the chlorine atom in the substrate turned out to be warrant of the positive result of the biotransformation process.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1993·International Journal of Systematic Bacteriology·W LudwigC G Friedrich
Feb 24, 2001·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·M O Ilori, D I Amund
Aug 15, 2001·Folia Microbiologica·N Dogra, G N Qazi
Jan 24, 2004·Applied Microbiology and Biotechnology·K YokoyamaY Kikuchi
Nov 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Pascal AuffingerP Shing Ho
Jan 16, 2007·Journal of Biotechnology·Gudrun Fischer-ColbrieGeorg Guebitz
Feb 23, 2008·Chemistry & Biology·Christopher S NeumannChristopher T Walsh
Feb 9, 2010·Journal of Hazardous Materials·Louisa Wessels Perelo
Jun 10, 2010·Biodegradation·Scarlet Nere OrtegaAndré Luiz Meleiro Porto
Nov 25, 2011·Enzyme and Microbial Technology·Witold GładkowskiCzesław Wawrzeńczyk
Dec 14, 2011·Marine Biotechnology·Lenilson C RochaAndré L M Porto
Feb 14, 2012·Environmental Science and Pollution Research International·Lin-Na DuYu-Hua Zhao
Feb 24, 2012·Systematic Biology·Fredrik RonquistJohn P Huelsenbeck
Aug 1, 2012·Nature Methods·Diego DarribaDavid Posada
Aug 16, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Małgorzata Grabarczyk
Nov 10, 2012·Applied Microbiology and Biotechnology·Julián Rafael DibFriedhelm Meinhardt
Oct 8, 2013·Aerobiologia·Krzysztof FrączekDariusz Ropek
Feb 18, 2015·Biotechnology Advances·Willian G BirolliAndré L M Porto
Feb 19, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Małgorzata GrabarczykMirosław Anioł
May 9, 2015·Applied Biochemistry and Biotechnology·Igor A Parshikov, John B Sutherland
Jul 15, 2015·Applied Microbiology and Biotechnology·Igor A ParshikovJohn B Sutherland
Mar 24, 2016·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Melissa Lau Moon LinL Lee Dupuis
Jan 21, 2017·Chemical Reviews·Vinayak AgarwalBradley S Moore
Feb 2, 2017·Pest Management Science·Peter Jeschke

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Datasets Mentioned

BETA
KY948067
KY948023
KY948068

Methods Mentioned

BETA
PCR
electrophoresis
column chromatography
NMR

Software Mentioned

jModelTest
Blast
MrBayes
Bioedit
Phyml

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