Salvianolic Acid A Inhibits OX-LDL Effects on Exacerbating Choroidal Neovascularization via Downregulating CYLD

Oxidative Medicine and Cellular Longevity
Ke MaoXingwei Wu

Abstract

Age-related macular degeneration is closely related to lipid oxidation, while relationship between OX-LDL and choroidal neovascularization is unclear. Recently, cylindromatosis is proved to regulate angiogenesis. However, its role in CNV progression remained unclear. Salvianolic acid A is widely used in vascular diseases. We investigated the relationship between OX-LDL and CNV and explore antineovascularization mechanism of Sal A. C57BL6/J mice were randomized into four groups and injected with PBS or OX-LDL, together with Sal A for one week. CNV was induced by laser; CNV severity was analyzed by fundus fluorescein angiography, H&E staining, and choroid flat mount after 1 week. In in vitro experiments, ARPE-19 and HUVECs were cultured with OX-LDL (with or without Sal A) for 48 hours. Angiogenic proteins, cell junction integrity, and tube formation were measured. CYLD siRNA and specific inhibitors were used to explore mechanisms of CYLD in promoting OX-LDL-induced CNV progression. OX-LDL promoted laser-induced CNV volume by increasing VEGF, PDGF, and CYLD levels. Sal A antagonized OX-LDL effects and restrained CNV progression by decreasing VEGF/PDGF/CYLD, increasing antiangiostatin levels, and promoting P62-CYLD-TRAF6 interactio...Continue Reading

References

Mar 3, 2005·Journal of Ethnopharmacology·Xin-Jian WangJian-Xing Xu
Oct 11, 2005·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Sandra CapraSusan Ash
Mar 29, 2007·Investigative Ophthalmology & Visual Science·Motohiro KameiShigeru Kinoshita
Jun 19, 2007·Biochemical and Biophysical Research Communications·Betty LamotheBryant G Darnay
Apr 3, 2008·The Journal of Clinical Investigation·Wei JinShao-Cong Sun
Oct 14, 2008·Progress in Retinal and Eye Research·Stuart G JarrettMichael E Boulton
Dec 17, 2009·Investigative Ophthalmology & Visual Science·Nilanjana SenguptaMaria B Grant
Feb 8, 2011·Investigative Ophthalmology & Visual Science·Daisuke TomidaHiroko Terasaki
Apr 22, 2011·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Marion BergmannJürgen Kopitz
Jun 10, 2011·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yuan-yuan ShiNico van Rooijen
Aug 16, 2011·International Immunopharmacology·Kwang-Seok OhByung Ho Lee
Mar 13, 2012·Biochemical and Biophysical Research Communications·Shuai LiuTaixing Cui
Apr 19, 2012·Molecular Aspects of Medicine·Stuart G Jarrett, Michael E Boulton
May 19, 2012·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Ren-An QinZhu-Ying Huang
Aug 5, 2014·BioMed Research International·Kuan-Chung ChenCalvin Yu-Chian Chen
Sep 3, 2016·International Journal of Ophthalmology·Ya-Jie YuWu Liu
Dec 8, 2016·Experimental Eye Research·Wan-Ju ChenElia J Duh
Dec 20, 2016·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Jiaxian GuoXiaodong Sun

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Methods Mentioned

BETA
electrophoretic methods
ELISA
transfection
Immunoprecipitation
PCR
ubiquitination
deubiquitination

Software Mentioned

image J
SigmaPlot
GraphPad Prism

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