Salvianolic acid B reverses multidrug resistance in nude mice bearing human colon cancer stem cells

Molecular Medicine Reports
Piaoting GuoYanhua Li

Abstract

Salvianolic acid B (SalB) is a water‑soluble phenolic compound, extractable from Salvia miltiorrhiza, and has previously been demonstrated to reverse tumor multidrug resistance (MDR) in colon cancer cells. Cancer stem cells (CSCs) are closely associated with drug resistance. Therefore, establishing a nude mouse model bearing human colon CSCs is important for the study of the mechanisms underlying colon cancer drug resistance as well as the reversal of drug resistance. The present study aimed to establish a nude mouse model bearing human colon CSCs and to investigate the effects of SalB on the drug resistance exhibited by the nude mouse model as well as determine its underlying mechanism. Cells from two colon cancer cell lines (LoVo and HCT‑116) were cultured in serum‑free medium to obtain CSCs‑enriched spheroid cells. Following this, nude mice were transplanted with LoVo and HCT‑116 colon CSCs to establish the CSC nude mouse model, which was subsequently demonstrated to exhibit MDR. The results of the present study revealed that following treatment with SalB, the chemotherapeutic drug resistance of xenografts was reversed to a certain extent. Western blot analysis was performed to investigate the expression levels of cluster of...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 28, 2009·Expert Opinion on Drug Metabolism & Toxicology·Yi An, Weg M Ongkeko
May 10, 2011·Current Colorectal Cancer Reports·Brian J WilsonNatasha Y Frank
Jun 16, 2011·International Journal of Molecular Sciences·Tao YinChunyou Wang
Feb 9, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Burkhard GreveMartin Götte
Jan 11, 2013·Thyroid : Official Journal of the American Thyroid Association·Valeria CarinaCarla Giordano
Nov 1, 2013·Molecular Medicine Reports·Lihui WeiJun Peng
Jan 13, 2015·Experimental and Therapeutic Medicine·Chong DI, Yaodong Zhao
Feb 20, 2016·Mini Reviews in Medicinal Chemistry·Jagat R KanwarRupinder K Kanwar
Apr 27, 2016·Cancer Science·Shoichiro Takeishi, Keiichi I Nakayama
Dec 22, 2017·Oncotarget·April B CabangMichael J Wargovich
Apr 6, 2018·Bioinformation·Saba HasanHalima El Omri

❮ Previous
Next ❯

Methods Mentioned

BETA
xenograft
flow cytometry
PCR
protein assay
xenografts

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.