Same-day confirmation of Staphylococcus aureus bacteraemia by a thermonuclease test

Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology
K Bergh, J A Maeland

Abstract

A test to detect thermostable DNase activity (TDNase test) was evaluated for rapid confirmation of S. aureus bacteraemia, using toluidine blue O DNA agar plates for the testing. A total of 226 blood cultures which grew bacteria were examined. S. aureus was identified in 76 of the cultures. All of the S. aureus isolates showed positive TDNase test after 2 h of incubation of the test plates. The remaining isolates examined, most of them coagulase-negative staphylococci, showed a negative test. The TDNase test enables reliable same-day confirmation of S. aureus bacteraemia.

References

Jun 1, 1985·Journal of Clinical Microbiology·H B Ratner, C W Stratton
Sep 1, 1983·Journal of Clinical Microbiology·B M Madison, V S Baselski
Nov 1, 1983·Journal of Clinical Microbiology·R Gudding

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