Same Difference? Low and High Glucosinolate Brassica rapa Varieties Show Similar Responses Upon Feeding by Two Specialist Root Herbivores

Frontiers in Plant Science
Rebekka SontowskiNicole M Van Dam

Abstract

Glucosinolates (GSLs) evolved in Brassicaceae as chemical defenses against herbivores. The GSL content in plants is affected by both abiotic and biotic factors, but also depends on the genetic background of the plant. Since the bitter taste of GSLs can be unfavorable for both livestock and human consumption, several plant varieties with low GSL seed or leaf content have been bred. Due to their lower GSL levels, such varieties can be more susceptible to herbivore pests. However, low GSL varieties may quickly increase GSL levels upon herbivore feeding by activating GSL biosynthesis, hydrolysis, or transporter genes. To analyze differences in herbivore-induced GSL responses in relation to constitutive GSL levels, we selected four Brassica rapa varieties, containing either low or high root GSL levels. Plants were infested either with Delia radicum or Delia floralis larvae. The larvae of both root flies are specialists on Brassica plants. Root samples were collected after 3, 5, and 7 days. We compared the effect of root herbivore damage on the expression of GSL biosynthesis (CYP79A1, CYP83B2), transporter (GTR1A2, GTR2A2), and GSL hydrolysis genes (PEN2, TGG2) in roots of low and high GSL varieties in conjugation with their GSL leve...Continue Reading

References

Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J W FaheyP Talalay
Aug 16, 2003·Journal of Chemical Ecology·Anurag A Agrawal, Nile S Kurashige
Apr 21, 2006·Phytochemistry·Atle M Bones, John T Rossiter
May 20, 2006·The Plant Journal : for Cell and Molecular Biology·Günter BraderE Tapio Palva
Jan 30, 2007·The Plant Journal : for Cell and Molecular Biology·Jae Hak Kim, Georg Jander
Sep 25, 2008·Annual Review of Entomology·Richard J HopkinsJoop J A van Loon
Dec 20, 2008·Science·Nicole K ClayFrederick M Ausubel
Mar 31, 2011·Journal of Chemical Ecology·Prisca S PierreSébastien Dugravot
Jan 1, 2010·The Arabidopsis Book·Ute Wittstock, Meike Burow
Mar 13, 2012·Phytochemistry·Niels Agerbirk, Carl Erik Olsen
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen
Jun 1, 2013·International Journal of Molecular Sciences·María Del Carmen Martínez-BallestaMicaela Carvajal
Nov 14, 2013·Molecular Ecology·Vartika MathurNicole M van Dam
Sep 12, 2014·Plant Physiology·Svend Roesen MadsenBarbara Ann Halkier
Mar 1, 2016·Frontiers in Plant Science·Wei ZhangDaniel J Kliebenstein
Mar 4, 2017·ELife·Morten Egevang JørgensenBarbara Ann Halkier
Apr 1, 2017·Journal of Visualized Experiments : JoVE·Katharina Grosser, Nicole M van Dam

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Citations

Aug 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sylvia MainaHo-Youn Kim

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BioRad CFX manager
R Core
Primer3web
Thermo Sientific Chromeleon Chromatography Data System
R

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