PMID: 7536671Dec 1, 1994Paper

Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension

Electrophoresis
T RabilloudJ J Lawrence

Abstract

We describe a modification in the sample application mode for isoelectric focusing with immobilized pH gradients. Instead of being applied at the surface of the gel in a sample cup, the sample is introduced into the gel during the immobilized pH gradient strip rehydration step. This modification implies the use of low percentage gels (below 3.5% T) and specially designed, but simple, rehydration chambers. The main advantages are a uniform resolution without side effects and the possibility of handling large sample volumes (500 microL for a standard 3 x 160 x 0.5 mm strip), allowing micropreparative work (milligram samples) with a simple experimental design.

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Citations

Nov 18, 2000·Electrophoresis·A JournetJ Garin
Jul 12, 2002·Proteomics·Paola RoncadaGian Franco Greppi
May 1, 1996·Electrophoresis·T Rabilloud
Mar 1, 1997·Electrophoresis·G L CorthalsA A Gooley
Apr 4, 1998·Electrophoresis·C PasqualiL A Huber
Jun 21, 2005·European Urology·Minoru SaitoKatsutoshi Yoshizato
Sep 11, 2002·Molecular Immunology·Ivar HordvikCurt Endresen
Mar 18, 1999·Molecular and Biochemical Parasitology·W YangD P McManus
Dec 30, 1999·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·S Harnisch, R H Müller
May 14, 1999·Drug Discovery Today·J H Wang, R M Hewick

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