PMID: 6412615Jul 1, 1983

Sample collection and processing for the assay of plasma renin activity. A comparison of anticoagulants and inhibitors

Annals of Clinical Biochemistry
J E RoulstonA L Muir


Plasma renin activity was measured in samples collected in lithium heparin, sodium heparin, and ethylenediaminetetraacetate. The samples were incubated under two widely used sets of conditions to generate angiotensin I, and the results were compared. Under certain incubation conditions, the absence of ethylenediaminetetraacetate from the sample tube produced low plasma renin activity values. This was obviated by using an alternative incubation medium and was not, therefore, due to direct inhibition of renin by heparin. These lower values were not seen in samples from patients receiving captopril. This suggests that the discrepant values were due to some angiotensin I conversion to angiotensin II and lower homologues in the incubates. Sodium heparin samples compared closely with lithium heparin samples, indicating that the presence of lithium is not significant. These results show that, provided the incubation conditions are suitably modified, samples collected into lithium or sodium heparin can be used for measurement of plasma renin activity.


Aug 15, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·J E Roulston, G A MacGregor
Jan 1, 1978·Nephron·P C ChurchillF D McDonald
Feb 19, 1972·Lancet·E E KritzingerJ J Jones
Jun 3, 1972·Lancet·J R StockigtE G Biglieri
May 1, 1967·The Journal of Clinical Endocrinology and Metabolism·J E SealeyJ H Laragh
Jul 1, 1971·Biochemical Pharmacology·D W Cushman, H S Cheung
Apr 1, 1972·Kidney International·J E SealeyJ H Laragh
Jul 1, 1981·Pathology·J R Stockigt, M J Hewett


Jan 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·C G WathenJ E Roulston
Nov 1, 1983·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·J E RoulstonC G Wathen

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