PMID: 9659274Jul 11, 1998Paper

Sample dilution to resolve mistaken identification of haemoglobin D as haemoglobin E using the variant automated system

Journal of Clinical Pathology
S Thomas

Abstract

High performance liquid chromatography (HPLC) is increasingly being used to estimate variant haemoglobins. A case of haemoglobin S/D (HbS/D) is presented, which was misdiagnosed as haemoglobin S/E (HbS/E) by HPLC. The patient was a 22 year old woman with sickle cell anaemia. Subsequent haemodilution by blood transfusion clearly elucidated the haemoglobin D peak on HPLC. Sample dilution experiments, using the pretransfusion sample, were done resulting in correct elution of the peak in the D window. Troubleshooting in similar problematic haemoglobin variant peaks seen on HPLC can be done by sample dilution.

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Citations

Dec 16, 2000·Journal of Neurosurgery·B P McKinleyR J Plunkett
Oct 12, 2005·Journal of Morphology·Alan FeducciaJ Richard Hinchliffe
Feb 1, 2007·Animal : an International Journal of Animal Bioscience·A L F HellwingA Skrede
Feb 8, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Lamia RebhiAli Bouslama
Apr 25, 2006·Virchows Archiv : an International Journal of Pathology·Rodolfo MontironiAldo V Bono
Jun 9, 2004·European Journal of Haematology·Vip ViprakasitVoravarn S Tanphaichitr

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