Sample displacement chromatography as a method for purification of proteins and peptides from complex mixtures.

Journal of Chromatography. a
Martina Srajer GajdosikDjuro Josic


Sample displacement chromatography (SDC) in reversed-phase and ion-exchange modes was introduced approximately twenty years ago. This method takes advantage of relative binding affinities of components in a sample mixture. During loading, there is a competition among different sample components for the sorption on the surface of the stationary phase. SDC was first used for the preparative purification of proteins. Later, it was demonstrated that this kind of chromatography can also be performed in ion-exchange, affinity and hydrophobic-interaction mode. It has also been shown that SDC can be performed on monoliths and membrane-based supports in both analytical and preparative scale. Recently, SDC in ion-exchange and hydrophobic interaction mode was also employed successfully for the removal of trace proteins from monoclonal antibody preparations and for the enrichment of low abundance proteins from human plasma. In this review, the principals of SDC are introduced, and the potential for separation of proteins and peptides in micro-analytical, analytical and preparative scale is discussed.


Jul 12, 1991·Journal of Chromatography·R S HodgesC T Mant
Jan 29, 1999·Journal of Chromatography. a·A Vailaya, C Horváth
May 9, 2000·Biotechnology and Bioengineering·A A ShuklaS M Cramer
Jan 11, 2001·Thrombosis Research·D JosićA Klukowska
Dec 19, 2002·Molecular & Cellular Proteomics : MCP·N Leigh Anderson, Norman G Anderson
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Djuro JosicAndrea Buchacher
Jul 9, 2004·Journal of Biochemical and Biophysical Methods·Even MansethRagnar Flengsrud
Jul 29, 2004·Biotechnology and Bioengineering·Fang XiaSteven M Cramer
Aug 20, 2004·Journal of Chromatography. a·Joachim ThiemannHartmut Schlüter
Oct 7, 2004·Current Opinion in Biotechnology·Alois JungbauerRobert Schlegl
Mar 24, 2005·Journal of Chromatography. a·Alois Jungbauer
Aug 8, 2006·Biotechnology Journal·Andrea Buchacher, Günter Iberer
Apr 3, 2007·Transfusion Medicine Reviews·Thierry Burnouf
Dec 14, 2007·Nature·Benjamin F CravattJohn R Yates
Feb 5, 2008·Journal of Chromatography. a·Alois Jungbauer, Rainer Hahn
Mar 11, 2008·Thrombosis Research·Peter AizawaGöran Karlsson
Jul 19, 2008·Biotechnology and Bioengineering·A KunduS M Cramer
Sep 17, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ji QiuSamir M Hanash
Apr 22, 2009·Biotechnology and Bioengineering·Anna M SenczukYinges Yigzaw
Apr 23, 2009·Molecular Oncology·Ralph SchiessRuedi Aebersold
Oct 27, 2009·Journal of Chromatography. a·Robert AhrendsHartmut Schlüter
May 26, 2010·Biotechnology and Applied Biochemistry·Arick BrownChris Dowd
Jul 14, 2010·Nature Biotechnology·Peter Mitchell
Sep 14, 2010·Journal of Chromatography. a·Christopher J MorrisonSteven M Cramer
Nov 10, 2010·Molecular BioSystems·Anna-Marja AuraKirsi-Marja Oksman-Caldentey
Dec 15, 2010·Journal of Proteome Research·Silvia SurinovaRuedi Aebersold
Jan 14, 2011·Nature·George Poste
Mar 25, 2011·Nature·Vicki Brower
Apr 29, 2011·Analytical Chemistry·Steven T EvansSteven M Cramer
Jun 21, 2011·Nature Biotechnology·Jeffrey R WhiteakerAmanda G Paulovich
Jun 28, 2011·Electrophoresis·Marijana RucevicDjuro Josic
Jul 6, 2011·Biotechnology Journal·Alois Jungbauer

❮ Previous
Next ❯


Oct 1, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniel A RibeiroLeda R Castilho
Jun 26, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Robert AhrendsHartmut Schlüter
Sep 9, 2015·Analytical Chemistry·Wonryeon Cho
Sep 1, 2015·Journal of Chromatography. a·Urh ČernigojAleš Štrancar
Sep 15, 2017·Electrophoresis·Uroš AndjelkovićMilica Popović
Oct 8, 2020·Biomedical Chromatography : BMC·Edouard C Nice
Dec 24, 2018·Journal of Chromatography. a·Ohnmar KhanalAbraham M Lenhoff
Sep 10, 2021·Electrophoresis·Egisto BoschettiPier Giorgio Righetti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.