Sample displacement chromatography as a method for purification of proteins and peptides from complex mixtures.

Journal of Chromatography. a
Martina Srajer GajdosikDjuro Josic

Abstract

Sample displacement chromatography (SDC) in reversed-phase and ion-exchange modes was introduced approximately twenty years ago. This method takes advantage of relative binding affinities of components in a sample mixture. During loading, there is a competition among different sample components for the sorption on the surface of the stationary phase. SDC was first used for the preparative purification of proteins. Later, it was demonstrated that this kind of chromatography can also be performed in ion-exchange, affinity and hydrophobic-interaction mode. It has also been shown that SDC can be performed on monoliths and membrane-based supports in both analytical and preparative scale. Recently, SDC in ion-exchange and hydrophobic interaction mode was also employed successfully for the removal of trace proteins from monoclonal antibody preparations and for the enrichment of low abundance proteins from human plasma. In this review, the principals of SDC are introduced, and the potential for separation of proteins and peptides in micro-analytical, analytical and preparative scale is discussed.

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Citations

Oct 1, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniel A RibeiroLeda R Castilho
Jun 26, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Robert AhrendsHartmut Schlüter
Sep 9, 2015·Analytical Chemistry·Wonryeon Cho
Sep 1, 2015·Journal of Chromatography. a·Urh ČernigojAleš Štrancar
Sep 15, 2017·Electrophoresis·Uroš AndjelkovićMilica Popović
Oct 8, 2020·Biomedical Chromatography : BMC·Edouard C Nice
Dec 24, 2018·Journal of Chromatography. a·Ohnmar KhanalAbraham M Lenhoff
Sep 10, 2021·Electrophoresis·Egisto BoschettiPier Giorgio Righetti

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