Sample displacement chromatography of Atlantic Salmon (Salmo salar) thrombin

Journal of Biochemical and Biophysical Methods
Even MansethRagnar Flengsrud

Abstract

A modified method of sample displacement chromatography (SDC) was used to purify active salmon thrombin on a heparin-coupled matrix to near homogeneity in milligram amounts from 117 ml plasma. This was achieved by combining a low-pressure multi-column affinity chromatography system with non-homogenous sample application in the order of increasing affinity to Heparin Sepharose. The results suggest that this modified method could be useful in protein purification. Some characteristics of salmon thrombin are presented.

References

Jul 12, 1991·Journal of Chromatography·R S HodgesC T Mant
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 15, 1989·Analytical Biochemistry·R Flengsrud, G Kobro
Jan 12, 2001·Thrombosis Research·L Z WangE S Sawyer

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Citations

Sep 1, 2015·Journal of Chromatography. a·Urh ČernigojAleš Štrancar
Apr 24, 2012·Journal of Chromatography. a·Martina Srajer GajdosikDjuro Josic

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