Sample manipulation and data assembly for robust microcrystal synchrotron crystallography

IUCrJ
Gongrui GuoQun Liu

Abstract

With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo-graphy with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.

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Citations

Apr 6, 2019·Acta Crystallographica. Section D, Structural Biology·Shibom BasuVincent Olieric
Jul 7, 2020·Acta Crystallographica. Section D, Structural Biology·Greta M AssmannKay Diederichs
Oct 6, 2018·Acta Crystallographica. Section D, Structural Biology·R Bruce DoakIlme Schlichting
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Mar 3, 2021·Journal of Synchrotron Radiation·Dieter K SchneiderMartin R Fuchs
May 6, 2021·Acta Crystallographica. Section D, Structural Biology·Gabrielle IllavaRobert E Thorne
Sep 3, 2021·Acta Crystallographica. Section D, Structural Biology·Isabelle MartielCelestino Padeste

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Methods Mentioned

BETA
macromolecular crystallography
X-ray
chips
transmission electron microscopy

Software Mentioned

AIMLESS
ANO
phenix
PEAKMAX
POINTLESS
CCP
BLEND
refine
DIALS
SmRmerge

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