Sample preparation, high-performance liquid chromatographic separation and determination of substance P-related peptides

Journal of Chromatography. B, Biomedical Applications
K Rissler

Abstract

This review deals with the determination of low levels of substance P and peptide fragments derived from the undecapeptide, i.e. covers the whole amount of so-called substance P-like immunoreactivity (SPLI) in biological samples. First an overview of the most currently used sample pretreatment procedures is given, followed by a description of the most effective high-performance liquid chromatographic (HPLC) separation methods. Special attention is paid to the choice of the appropriate column and the possible pitfalls encountered in separation of fmol amounts of peptide material. Subsequently the most important techniques of detection are discussed. This section primarily focuses on the coupling of HPLC with radioimmunoassay (RIA), which is indispensable for detection of components in the fmol range at present. Finally, some aspects of preparation and chromatographic separation of radiolabelled antigens for use in RIA are discussed.

References

Jan 1, 1979·Biochemical Pharmacology·C M LeeL L Iversen
Nov 1, 1979·Biochemical Pharmacology·H BergerH Niedrich
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N V Costrini, R A Bradshaw
Dec 1, 1979·Hoppe-Seyler's Zeitschrift für physiologische Chemie·C B HewardV J Hruby
Sep 12, 1979·Biochemical and Biophysical Research Communications·S Blumberg, V I Teichberg
Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Jul 15, 1978·FEBS Letters·E Heymann, R Mentlein
Oct 1, 1978·Naunyn-Schmiedeberg's Archives of Pharmacology·F LembeckR Gamse
Jan 1, 1976·Annual Review of Biochemistry·M Morrison, G R Schonbaum
Jun 15, 1992·European Journal of Biochemistry·J Jensen, J M Conlon
Dec 1, 1991·Journal of Applied Physiology·J Solway, A R Leff
Aug 1, 1991·Immunology and Cell Biology·A EglezosR D Helme
Sep 1, 1990·Cellular and Molecular Neurobiology·M Otsuka, M Yanagisawa
Mar 1, 1991·The American Review of Respiratory Disease·P J Barnes
Jan 1, 1991·International Archives of Allergy and Applied Immunology·P J Barnes
Oct 1, 1991·Clinical and Experimental Pharmacology & Physiology·D Stretton
Mar 1, 1991·The American Review of Respiratory Disease·J G Widdicombe
May 31, 1991·Biochemical and Biophysical Research Communications·H KozawaH Matsuo
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F NybergL Terenius
Jun 28, 1985·Biochemical and Biophysical Research Communications·S EndoS Ishii

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Citations

Sep 2, 2008·Analytical and Bioanalytical Chemistry·Anna Poliwoda, Piotr P Wieczorek
Dec 29, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Takanori Karasawa, Masao Sugawara
Dec 20, 2005·Cellular Immunology·Daniel A Nelson, Kenneth L Bost
Jan 6, 2004·Cornea·Masakazu YamadaTeruo Nishida
Sep 13, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Misato SakamotoMasao Sugawara
Sep 15, 2006·Clinical and Vaccine Immunology : CVI·Donald E CampbellSteven D Douglas
Feb 6, 2009·Clinical and Vaccine Immunology : CVI·Donald E CampbellSteven D Douglas
Jun 15, 1997·Analytical Chemistry·D J AndersonK A Davis

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